Molecular Biology Course Based on Study of Transcriptional Control

生物科学（61）该项目的目的是建立一个基本的分子生物学实验室，在分子生物学新课程的背景下，将研究性实验纳入课程。该项目旨在将NSF支持的“酵母实验课堂指南”改编为一个有趣的未知研究问题，作为分子生物学学生的起点。实验方法为学生提供关键分子生物学协议的实践培训，这些协议适用于有关转录控制和重要分子进化保守的研究问题。分子生物学实验室的设计方式，使学生能够分析基因在三个基本水平，即DNA，RNA和蛋白质，使用体外和体内的方法，以及产生学生的学习成果。该课程强调了理解方法，分子方法和仪器的重要性，这些方法和仪器已经彻底改变了生物学，是诊断和预防疾病的关键，并允许学生讨论其中一些新技术带来的伦理问题。正在收购的设备也被用于改善其他生物学课程的实验室组成部分，即遗传学，细胞生理学和生物化学，从而使UOG的生物学课程现代化。在作为普通教育要求提供的非主要人类生物学课程中，正在使用PCR热循环仪和凝胶电泳仪等具体设备，以展示分子生物学在法医学、亲子鉴定案件、人类学和考古学调查中的应用。

Biological Sciences (61) The aim of this project is to establish a basic molecular biology lab that, in the context of a new course in Molecular Biology, will implement research-based experiments into the curriculum. The project intends to adapt the NSF-supported "Classroom Guide to Yeast Experiments" to an interesting, unknown research question that serves as a starting point for molecular biology students. The experimental approaches provide students with hands-on training in key molecular biology protocols that are applied to a research problem regarding transcriptional control and the evolutionary conservation of important molecules. The Molecular Biology lab is designed in a manner that enables students to analyze genes at three fundamental levels, namely, DNA, RNA, and Proteins, using both in vitro and in vivo approaches, as well as produce student learning outcomes. The course emphasizes the importance of understanding methodologies, molecular approaches, and instrumentation that have revolutionized biology and are key to diagnosis and prevention of disease, as well as allow the students to debate the ethical considerations that some of these new technologies pose. The equipment being acquired is also being used to improve the laboratory component of other biology courses, namely Genetics, Cell Physiology, and Biochemistry, thereby modernizing the Biology curriculum at UOG. Specific equipment like the PCR Thermal Cycler and gel electrophoresis apparatus is being used for the non-major Human Biology course offered as a general education requirement, in order to demonstrate applications of molecular biology in forensic sciences, paternity cases, anthropological and archaeological investigations.