The Molluscan Mitochondrial Genomic Project: Phylogenetic Analysis, Evaluation and Enrichment

david R. lindberg博士。加州大学伯克利分校的大卫·r·林德伯格（David R. Lindberg）获得了一笔资金，用于对50种腹足类软体动物的全线粒体基因组进行测序。虽然腹足类动物的进化史已经被研究了很长时间，并且有很好的化石记录，但由于形态趋同和先前分析的基因信息不足，人们对腹足类动物树底部的分支模式知之甚少。本项目将研究线粒体基因序列，这是软体动物的一个有前途的新数据集，并利用这些数据重建腹足动物内部以及腹足动物与其他软体动物之间的关系。在过去，线粒体基因组的完整测序非常耗时，很少有软体动物被研究过。然而，随着几个主要测序计划的完成，高通量测序设施正在变得可用。该项目将使用劳伦斯伯克利国家实验室的高通量果蝇基因组测序中心，并由美国、德国和澳大利亚的研究人员合作分析数据。该项目的具体目标包括鉴定、分析和评估基因、rRNA和tRNA在线粒体基因组中的位置、二级结构和重建关系的序列。化石记录还将用于关联首次出现的数据，评估不同腹足类动物群体中每个基因的解析潜力，并为目前具有暗示意义的宏观进化模式提供重要见解，但由于我们目前对最早的腹足类软体动物之间的关系缺乏了解，这些模式受到限制。它还将为无数无脊椎动物学家、分子生物学家、生物信息学家和分子进化论者提供初步和探索性的数据，并将作为一个试点项目，用于评估与高通量测序中心（如劳伦斯伯克利和其他国家实验室的高通量测序中心）的合作关系，并探索它们在重建生命之树中的潜在作用。

DEB-0089624David R. LindbergDr. David R. Lindberg at the University of California at Berkeley has received funding to sequence complete mitochondrial genomes of 50 species of gastropod mollusks. Although the evolutionary history of gastropods has been long studied and the group has an excellent fossil record, the branching patterns at the base of the gastropod tree are poorly understood because of morphological convergence and the uninformative nature of the genes that have been previously analyzed. This project will examine mitochondrial gene order, a promising new data set for mollusks, and use these data to reconstruct the relationships within the Gastropoda and between gastropods and other mollusks. In the past complete sequencing of mitochondrial genomes has been so time consuming that few mollusks have been studied. However, with the completion of several major sequencing initiatives, high-throughput sequencing facilities are becoming available. This project will use the high-throughput Drosophila Genome Sequencing Center at Lawrence Berkeley National Laboratory, and the data analyzed by a collaboration of researchers in the USA, Germany and Australia. Specific aims of this project include the identification, analysis and evaluation of gene, rRNA, and tRNA placement within the mitochondrial genome, secondary structures, and sequences for reconstructing relationships. The fossil record will also be used to correlate first appearance data and evaluate the resolution potential of each gene in different gastropod groups, and provide important insights into macroevolutionary patterns that are currently suggestive, but limited by our current lack of understanding of the relationships among the earliest gastropod molluscs. It will also provide preliminary and exploratory data for countless invertebrate zoologists, molecular biologists, bioinformaticists, and molecular evolutionists, and will serve as a pilot project for the evaluation of partnerships with high-throughput sequencing centers such as those housed at Lawrence Berkeley and other National Laboratories, and explores their potential role in reconstructing the Tree of Life.