Freshwater plankton as a habitat for hygienically relevant microorganisms

表层沃茨中的浮游生物提供了一个可以被生物膜定殖的大表面。这些可以窝藏可由浮游生物作为载体运输的具有生物学意义的微生物。这已被证明是弧菌物种，但对其他生物和淡水知之甚少，而第一次的结果表明了类似的情况。此外，微生物可以进入活的但不可培养（VBNC）状态，并且不会通过常规培养方法检测到。当水用于饮用水生产和/或娱乐目的时，这会造成卫生风险。在这个项目中，殖民地（活的和死的）当地典型的浮游植物和浮游动物的目标生物将在中营养湖（Baldeneysee）进行调查。将根据可能的输入部位和目标细菌事件进行定义的时空采样，采样点为采样点。目标微生物是粪便指示物（E。coli、肠球菌、C.产气荚膜杆菌）和病原体（弯曲杆菌属，铜绿假单胞菌、军团菌属和气单胞菌属（Aeromonas spp.）特别注意的是支付VBNC状态，将使用分子生物学方法检测。将通过培养以及直接活菌计数、膜完整性测定、FISH和实时PCR测定军团菌属的活力。该项目的结果将有助于风险评估。

Plankton in surface waters provides a large surface which can be colonized by biofilms. These can harbour hygienically relevant microorganisms which can be transported by plankton organisms as vectors. This has been shown for Vibrio species, but much less is known about other organisms and for freshwater, while first results indicate a similar situation. Furthermore, the organisms can enter a viable but non culturable (VBNC) state and will not be detected by conventional culture methods. This poses a hygienic risk when the water is used for drinking water production and/or for recreational purposes. In this project, colonization of both (living and dead) locally typical phytoplankton and Zooplankton by target organisms will be investigated in a mesotrophic lake (Baldeneysee). Defined spatio-temporal sampling with sampling points according to possible input sites and events of target bacteria will be performed. Target organisms are fecal indicators (E. coli, enterococci, C. perfringens) and pathogens (Campylobacter spp., P. aeruginosa, Legionella spp. and Aeromonas spp.) Particular attention is payed to the VBNC state which will be detected using molecular biological methods. Viability will be determined by cultivation as well as by direct viable count, membrane integrity determination, FISH and real-time PCR for Legionella spp. Results of this project will contribute to risk assessment.