Novel Optical Biosensors for Hazardous Ions

用于有害离子的新型光学生物传感器

• 批准号: 0097442
• 负责人: Richard Thompson
• 金额: $ 41.81万
• 依托单位: University of Maryland at Baltimore
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0097442&HistoricalAwards=false
• 关键词: Novel; Optical; Biosensors; Hazardous; Ions

0097442ThompsonThe objective of this research is the development of new fluorescence-based sensors with high sensitivity and accuracy for important industrial analytes for which current chemical sensors are inadequate or nonexistent. In earlier work the investigators demonstrated that carbonic anhydrase-based biosensors were capable of transducing the levels of certain transition metals as changes in fluorescence intensity, lifetime, and anisotropy. These sensors were able to rapidly and continuously determine transition metal ions such copper and zinc at sub-part per billion levels in the presence of calcium and magnesium at million-fold higher concentrations. In this research the metal ion approach will be extended for the detection of the heavy metals lead, mercury and cadmium. The bulk of the engineering effort in this phase of the research will occur at the molecular level, in the construction of the fluorescent transducer/recognition molecules and determination of their properties. In addition, a previously developed approach, which uses fluorescence lifetime to detect anions such as cyanide and cyanate, will be adapted for the determination of the anions chromate, arsenate, and nitrate.

0097442爱普生本研究的目的是开发新的基于荧光的传感器，该传感器对于重要的工业分析物具有高灵敏度和准确度，而当前的化学传感器对于这些分析物是不充分的或不存在的。 在早期的工作中，研究人员证明了基于碳酸酐酶的生物传感器能够将某些过渡金属的水平转换为荧光强度，寿命和各向异性的变化。 这些传感器能够快速和连续地确定过渡金属离子，如铜和锌，在十亿分之几的水平，在存在的钙和镁在百万倍更高的浓度。 本研究将金属离子法推广到重金属铅、汞、镉的检测。 在这一阶段的研究中，大部分的工程工作将发生在分子水平上，在荧光转换器/识别分子的构建和确定其特性。 此外，以前开发的方法，使用荧光寿命来检测阴离子，如氰化物和氰酸根，将适用于测定的阴离子铬酸盐，砷酸盐，硝酸盐。