Upgrade to X-Ray Diffraction Facility

升级至 X 射线衍射设备

• 批准号: 0100284
• 负责人: Lesa Beamer
• 金额: $ 4.2万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-15 至 2003-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0100284&HistoricalAwards=false
• 关键词: Upgrade; Ray; Diffraction; Facility

This award provides partial support for purchase of a cryo-cooling device to be installed in a shared facility used for x-ray protein crystallography. Crystallographic studies now play a central role in biology as the sequencing of large stretches of DNA reveals more and more genes whose role in the cell are poorly understood. Cryo-cooling minimizes radiation damage to protein crystals during data collection, and is an essential feature of modern x-ray crystallography workstations. At least 90% of the current crystallography projects on campus require cryo-cooling, including structural studies of a thermostable glyceraldehyde-3-phosphate dehydrogenase, other NAD binding enzymes, human bactericidal/permeability-increasing protein, EF-hand calcium binding proteins, anti-DNA antibodies, and enzymes in the alginate biosynthetic pathway. Although one such device is already available to the PIs, usage of the X-ray facility is expected to increase significantly as a result of the recent establishment of a Structural Biology Core facility for which they are responsible.

该合同为购买低温冷却设备提供部分支持，该设备将安装在用于X射线蛋白质结晶学的共享设施中。结晶学研究现在生物学中发挥着核心作用，因为对大段DNA的测序揭示了越来越多的基因，这些基因在细胞中的作用尚不清楚。低温冷却在数据收集过程中将对蛋白质晶体的辐射损害降至最低，是现代X射线晶体工作站的基本功能。目前校园中至少90%的结晶学项目需要冷冻，包括耐热甘油醛-3-磷酸脱氢酶、其他NAD结合酶、人类杀菌/通透性增加蛋白、EF-手钙结合蛋白、抗DNA抗体以及藻酸盐生物合成途径中的酶的结构研究。尽管私人投资机构已经有一台这样的设备可用，但由于最近建立了一个由他们负责的结构生物学核心设施，预计X光设施的使用量将大幅增加。