Upgrade of in-house X-ray diffraction equipment

内部X射线衍射设备升级

• 批准号: 7040978
• 负责人: Seth A. Darst
• 金额: $ 33万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7040978
• 关键词: X ray; X ray crystallography; housing; lead; structural biology; university

DESCRIPTION (provided by applicant): " At The Rockefeller University, there are three relatively large, highly successful and productive structural biology groups that use X-ray crystallography as a major tool (Darst, structural biology of bacterial transcription; MacKinnon, structural biology of ion channels; Stebbins, structural biology of bacterial pathogenesis). In the last 5 years or so, three additional groups at Rockefeller have developed significant X-ray crystallography programs (Blobel, mRNA nuclear export; Rice, viral replication; Sakmar, G protein-coupled signalling). These efforts began as formal or informal collaborations with the structural biologists at Rockefeller, but in each case success has led to the expansion of these efforts, such that these groups support X-ray crystallography programs as large and active as the structural biologists. This situation has led to a need for significant upgrades of the in-house X-ray equipment at The Rockefeller University. Specifically, this Shared Instrumentation Grant proposes the following upgrades: 1) An R-Axis IV++ area detector to replace a 12-year-old R-Axis II area detector that will no longer be supported by MSC; 2) Inverse <)> brackets to add to two ports of the X-ray generator; 3) Two VariMax Cu HR confocal Max-Flux optical systems, the most advanced X-ray optics system available from Rigaku/MSC, to replace 12-year-old MSC/Yale mirrors; 4) Two X-stream cryogenic systems to replace obsolete, 15-year-old MSC cryosystems that are out of repair. These upgrades will significantly enhance the use of the machine for screening weakly diffracting crystals of membrane proteins and large macromolecular complexes, enhance data quality and throughput, as well as greatly facilitate its use for the screening and preparation of cryo-preserved crystals for synchrotron trips, which is the main use of the machines for a number of NIH-supported projects.

描述（由申请人提供）：“在洛克菲勒大学，有三个相对较大的，非常成功和富有成效的结构生物学小组，使用X射线晶体学作为主要工具（Darst，细菌转录的结构生物学; MacKinnon，离子通道的结构生物学; Stebbins，细菌发病机理的结构生物学）。在过去5年左右的时间里，洛克菲勒的另外三个小组开发了重要的X射线晶体学项目（Blobel，mRNA核输出; Rice，病毒复制; Sakmar，G蛋白偶联信号）。这些努力开始于与洛克菲勒的结构生物学家的正式或非正式合作，但在每一种情况下，成功都导致了这些努力的扩大，这些团体支持与结构生物学家一样大而活跃的X射线晶体学计划。这种情况导致需要对洛克菲勒大学的内部X射线设备进行重大升级。具体来说，这项共享仪器赠款提出了以下升级：1）一个R轴IV++区域探测器，以取代12岁的R轴II区域探测器，将不再由MSC支持; 2）反向<）>括号添加到X射线发生器的两个端口; 3）两个VariMax Cu HR共焦Max-Flux光学系统，理学/MSC最先进的X射线光学系统，以取代12年前的MSC/Yale反射镜; 4）两个X-stream低温系统，以取代过时的，15岁的MSC低温系统，是出于维修。这些升级将显着提高机器用于筛选膜蛋白和大分子复合物的弱衍射晶体的使用，提高数据质量和吞吐量，并大大促进其用于筛选和制备冷冻保存晶体的同步加速器旅行，这是机器的主要用途一些NIH支持的项目。