Glucosinolate hydrolysis in plants - the role of specifier proteins

植物中芥子油苷的水解 - 特异性蛋白的作用

• 批准号: 150734515
• 负责人: Professorin Dr. Ute Wittstock
• 金额: --
• 依托单位: Institut für Pharmazeutische Biologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/150734515?language=en
• 关键词: Glucosinolate; hydrolysis; plants; role; specifier

The diversity of the glucosinolate-myrosinase system, an activated chemical defense of the Brassicaceae and related families, arises from the variation of both glucosinolate biosynthesis and glucosinolate hydrolysis. Depending on the glucosinolate structure and the presence of certain protein factors, the specifier proteins, one glucosinolate can be hydrolyzed to different products with diverse biological activities. The goal of our project is to learn to understand, how and why plants generate diversity at the level of glucosinolate hydrolysis. The proposed project is aimed at exploring the biological role, regulation and localization of specifier proteins in A. thaliana, at elucidating the biochemical mechanism of specifier proteins based on the investigation of their structure and their interaction with myrosinase, and at studying how the protein family has evolved in glucosinolate-containing plants.

芥子油苷-黑芥子酶系统是菊科及其相关科植物的一种活性化学防御系统，其多样性来源于芥子油苷生物合成和芥子油苷水解的差异。根据芥子油苷的结构和某些蛋白质因子的存在，一种芥子油苷可以水解成具有不同生物活性的不同产物。我们项目的目标是了解植物如何以及为什么在芥子油苷水解水平上产生多样性。本项目的目的是探索A. thaliana的研究，在研究其结构及其与黑芥子酶的相互作用的基础上阐明特异性蛋白的生化机制，并研究蛋白质家族如何在含芥子油苷的植物中进化。

项目成果

Chapter Six – Glucosinolate Breakdown

• DOI: 10.1016/bs.abr.2016.06.006
• 发表时间: 2016-12
• 期刊: Advances in Botanical Research
• 作者: U. Wittstock;E. Kurzbach;Anna-Maria Herfurth;E. Stauber

Molecular models and mutational analyses of plant specifier proteins suggest active site residues and reaction mechanism

• DOI: 10.1007/s11103-013-0126-0
• 发表时间: 2014-01-01
• 期刊: PLANT MOLECULAR BIOLOGY
• 影响因子: 5.1
• 作者: Brandt, Wolfgang;Backenkoehler, Anita;Wittstock, Ute
• 通讯作者: Wittstock, Ute

The crystal structure of the thiocyanate-forming protein from Thlaspi arvense, a kelch protein involved in glucosinolate breakdown

• DOI: 10.1007/s11103-015-0351-9
• 发表时间: 2015-09-01
• 期刊: PLANT MOLECULAR BIOLOGY
• 影响因子: 5.1
• 作者: Gumz, Frauke;Krausze, Joern;Wittstock, Ute
• 通讯作者: Wittstock, Ute

Glucosinolate Breakdown in Arabidopsis: Mechanism, Regulation and Biological Significance

• DOI: 10.1199/tab.0134
• 发表时间: 2010
• 作者: U. Wittstock;Meike Burow

Structural diversification during glucosinolate breakdown: mechanisms of thiocyanate, epithionitrile and simple nitrile formation

• DOI: 10.1111/tpj.14327
• 发表时间: 2019-07-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Eisenschmidt-Boenn, Daniela;Schneegans, Nicola;Brand, Wolfgang
• 通讯作者: Brand, Wolfgang