Functional Analysis of the BAS1 Gene and Its Product: CYP 72B1
BAS1基因及其产物CYP 72B1的功能分析
基本信息
- 批准号:0114726
- 负责人:
- 金额:$ 36万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-08-15 至 2005-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Brassinosteroids are growth-promoting hormones involved in modulating plant plasticity in response to changes in the environment. Previous studies of brassinosteroids have concentrated on either the metabolic or reception pathways for these hormones. A novel gene affecting brassinosteroid responses in plants, CYP72B1, has recently been identified. This gene appears to be involved in brassinosteroid inactivation rather than biosynthesis or perception acting as a brassinosteroid hydroxylase that catabolizes the most active form of the hormone, brassinolide, into an inactive form, 26-hydroxybrassinolide. By regulating the active levels of brassinolide in certain tissues or at certain times, CYP72B1 may be acting as a modulator of brassinosteroid responses during plant development. One hypothesis is that CYP72B1 uses brassinolide as an enzyme substrate generating the less-active brassinosteroid, 26-hydroxybrassinolide. Heterologous expression of CYP72B1 in yeast coupled with feeding experiments using radiolabeled brassinolide will test whether CYP72B1 uses brassinolide as an enzyme substrate. Physiological analysis will also be used to further characterize 26-hydroxybrassinolide activity. A second hypothesis is that CYP72B1 expression affects growth in Arabidopsis by regulating brassinolide levels. A T-DNA knockout mutation in the CYP72B1 gene will allow a detailed analysis of the role this gene plays in development. A third hypothesis is that CYP72B1 activity is regulated transcriptionally and post translationally at the tissue specific level. RT-PCR experiments show that CYP72B1 transcripts accumulate greater in the rosettes vsersus the hypocotyls of developing seedlings. CYP72B1 transcript accumulation measurements will be expanded to many different tissues of both juvenile and adult plants. CYP72B1 translational fusions with reporter genes will also be used to examine CYP72B1 protein levels in different tissues during plant development. Together, this research will increase the understanding of CYP72B1's role in modulating brassinosteroid levels and perception during plant development. In addition to the training of one graduate student and one postdoctoral fellow, several undergraduates are likely to participate in this project. Findings from this research will be incorporated, when appropriate, into an undergraduate laboratory course on DNA manipulation taught by the Principal Investigator, Professor Michael Neff.
油菜素内酯是一种促进生长的激素,参与调节植物的可塑性以应对环境的变化。以前对油菜素内酯的研究主要集中在这些激素的代谢或接受途径上。最近发现了一个影响植物油菜素内酯反应的新基因CYP72B1。该基因似乎参与了油菜素内酯的失活,而不是生物合成或感知,作为油菜素内酯羟化酶,将最活跃的激素——油菜素内酯分解为不活跃的形式——26-羟基油菜素内酯。CYP72B1通过调节特定组织或特定时间的油菜素内酯活性水平,可能在植物发育过程中作为油菜素内酯反应的调节剂。一种假设是CYP72B1使用油菜素内酯作为酶底物产生活性较低的油菜素内酯26-羟基油菜素内酯。CYP72B1在酵母中的异源表达结合放射性标记油菜素内酯的饲养实验,将检测CYP72B1是否以油菜素内酯为酶底物。生理分析也将用于进一步表征26-羟基油菜素内酯活性。第二个假设是CYP72B1表达通过调节油菜素内酯水平影响拟南芥的生长。CYP72B1基因的T-DNA敲除突变将允许详细分析该基因在发育中所起的作用。第三种假设是CYP72B1活性在组织特异性水平上受转录和翻译后调节。RT-PCR实验表明,CYP72B1转录本在莲座中的积累量大于发育中的下胚轴。CYP72B1转录物积累的测量将扩展到许多不同的幼体和成年植物的组织。CYP72B1与报告基因的翻译融合也将用于检测植物发育过程中不同组织中CYP72B1蛋白的水平。总之,这项研究将增加对CYP72B1在植物发育过程中调节油菜素内酯水平和感知中的作用的理解。除了培养一名研究生和一名博士后外,还有几名本科生可能参与该项目。在适当的时候,这项研究的结果将被纳入由首席研究员Michael Neff教授教授的DNA操作本科实验课程。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Michael Neff其他文献
Storytelling Agents with Personality and Adaptivity
具有个性和适应性的讲故事代理
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
Chao Hu;M. Walker;Michael Neff;J. E. Tree - 通讯作者:
J. E. Tree
se-atlas: Mapping of Health Care Providers for People With Rare Diseases - a Cross-sectional Survey From the User Perspective
se-atlas:罕见病患者医疗保健提供者地图 - 从用户角度进行的横断面调查
- DOI:
10.21203/rs.3.rs-829482/v1 - 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
Jannik Schaaf;Michael Neff;Manuela Till;Niels Tegtbauer;H. Storf - 通讯作者:
H. Storf
Hand Gesture Synthesis for Conversational Characters
会话角色的手势合成
- DOI:
- 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
Michael Neff - 通讯作者:
Michael Neff
Predictive Feedback for Interactive Control of Physics‐based Characters
基于物理的角色交互控制的预测反馈
- DOI:
10.1111/j.1467-8659.2005.00850.x - 发表时间:
2005 - 期刊:
- 影响因子:2.5
- 作者:
Joseph Laszlo;Michael Neff;Karan Singh - 通讯作者:
Karan Singh
Modeling Relaxed Hand Shape for Character Animation
为角色动画建模放松的手形
- DOI:
- 发表时间:
2006 - 期刊:
- 影响因子:0
- 作者:
Michael Neff;H. Seidel - 通讯作者:
H. Seidel
Michael Neff的其他文献
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{{ truncateString('Michael Neff', 18)}}的其他基金
EAGER: Building a Foundation for Hands-on STEM Learning at a Distance: Pedagogical Agents for Embodied Education in Virtual Reality
EAGER:为远程实践 STEM 学习奠定基础:虚拟现实中实体教育的教学代理
- 批准号:
2232066 - 财政年份:2022
- 资助金额:
$ 36万 - 项目类别:
Standard Grant
EAGER: Collaborative Research: Interactive Dialog Agents for Social Language Development and Listening Comprehension
EAGER:协作研究:用于社交语言发展和听力理解的交互式对话代理
- 批准号:
1748058 - 财政年份:2017
- 资助金额:
$ 36万 - 项目类别:
Standard Grant
The ATAF2 Transcription Factor, Brassinosteroid Catabolism and Plant Development
ATAF2 转录因子、油菜素类固醇分解代谢和植物发育
- 批准号:
1656265 - 财政年份:2017
- 资助金额:
$ 36万 - 项目类别:
Continuing Grant
EXP: Collaborative Research: Gesture Enhancement of Virtual Agent Mathematics Tutors
EXP:协作研究:虚拟代理数学导师的手势增强
- 批准号:
1320029 - 财政年份:2013
- 资助金额:
$ 36万 - 项目类别:
Standard Grant
The Role Of Brassinosteroid Inactivation In Plant Development
油菜素类固醇失活在植物发育中的作用
- 批准号:
1124749 - 财政年份:2012
- 资助金额:
$ 36万 - 项目类别:
Continuing Grant
HCC: Small: Collaborative Research: Gestural and Linguistic Expressivity and Entrainment in Dialogue
HCC:小型:协作研究:手势和语言表达以及对话中的夹带
- 批准号:
1115872 - 财政年份:2011
- 资助金额:
$ 36万 - 项目类别:
Continuing Grant
CAREER: Generative Models for Character Animation and Gesture in the New Age of Art and Electronic Interaction
职业:艺术和电子交互新时代的角色动画和手势生成模型
- 批准号:
0845529 - 财政年份:2009
- 资助金额:
$ 36万 - 项目类别:
Standard Grant
Pilot: Increasing Creative Exploration with Computer Tools that Support Spontaneity & Embodiment
试点:利用支持自发性的计算机工具增加创造性探索
- 批准号:
0856084 - 财政年份:2009
- 资助金额:
$ 36万 - 项目类别:
Standard Grant
The Role of Brassinosteroid Inactivation in Plant Development
油菜素类固醇失活在植物发育中的作用
- 批准号:
0758411 - 财政年份:2007
- 资助金额:
$ 36万 - 项目类别:
Standard Grant
The Role of Brassinosteroid Inactivation in Plant Development
油菜素类固醇失活在植物发育中的作用
- 批准号:
0616153 - 财政年份:2006
- 资助金额:
$ 36万 - 项目类别:
Standard Grant
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