Acquisition of Instrumentation for Marine Genomics and Proteomics

购置海洋基因组学和蛋白质组学仪器

• 批准号: 0115801
• 负责人: Ronald Burton
• 金额: $ 25.4万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0115801&HistoricalAwards=false
• 关键词: Acquisition; Instrumentation; Marine; Genomics; Proteomics

#0115801, "Acquisition of instrumentation for marine genomics and proteomics." (NSF Major Research Instrumentation Program). PI: R.S. Burton ABSTRACT:A grant has been awarded to Dr. Burton at Scripps Institution of Oceanography (SIO) for the acquisition of instrumentation for analyses of genes and their expression in marine organisms. The instrumentation will enable researchers to study genome-wide responses to environmental stress at both the nucleic acid and protein levels and will form a core facility for use in the research projects of ten different laboratories at SIO. Although the specific research goals vary with the laboratory, the primary questions motivating the projects are similar: What are the molecular mechanisms by which organisms adapt to specific marine environments? What impact do these organismal adaptations have on ecosystem function? Specific research projects will consist of analyses of microbial communities in the ocean, including both quantitative identification of community members and functional composition of the communities. Both free-living microbes and those involved in symbiotic relationships with invertebrates will be analyzed with a focus on adaptations to temperature, pressure and nutrient regimes. Other studies will address interactions between components of the nuclear and mitochondrial genomes of invertebrate species and signal transduction pathways across marine invertebrate cell membranes. In addition to enabling multiple specific research projects, this grant will facilitate SIO's mission of training the next generation of ocean scientists. SIO students already take a variety of genomics classes and seminars across the entire UCSD campus; yet without easy access and hands-on training using modern instrumentation, students have been unable to take full advantage of existing technologies in designing their own dissertation research programs. This grant will enhance the research and training environment at SIO so that the institution will continue to attract top faculty, postdoctoral researchers and graduate students and provide a state-of-the-art research environment.

#0115801，“购置海洋基因组学和蛋白质组学仪器。（NSF重大研究仪器计划）。 PI：R.S.伯顿摘要：斯克里普斯海洋研究所（SIO）的伯顿博士获得了一笔赠款，用于购买分析海洋生物基因及其表达的仪器。 该仪器将使研究人员能够在核酸和蛋白质水平上研究全基因组对环境压力的反应，并将形成一个核心设施，用于SIO十个不同实验室的研究项目。 虽然具体的研究目标因实验室而异，但激发这些项目的主要问题是相似的：生物体适应特定海洋环境的分子机制是什么？ 这些生物的适应性对生态系统功能有什么影响？具体的研究项目将包括分析海洋中的微生物群落，包括从数量上确定群落成员和群落的功能组成。 无论是自由生活的微生物和那些参与共生关系与无脊椎动物将进行分析，重点是适应温度，压力和营养制度。 其他研究将涉及无脊椎动物物种的核和线粒体基因组成分之间的相互作用以及海洋无脊椎动物细胞膜上的信号转导途径。除了使多个具体的研究项目，这笔赠款将促进SIO的使命培训下一代海洋科学家。 SIO的学生已经在整个UCSD校园参加了各种基因组学课程和研讨会;然而，如果没有使用现代仪器的方便访问和实践培训，学生们就无法充分利用现有技术来设计自己的论文研究项目。 这笔赠款将加强在SIO的研究和培训环境，使该机构将继续吸引顶尖的教师，博士后研究人员和研究生，并提供一个国家的最先进的研究环境。