权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Collaborative Research: Molecular Basis Of Novel Phenotypes Resulting From Interpopulation Hybridization

合作研究：群体间杂交产生的新表型的分子基础

基本信息

批准号：
1556466
负责人：
Ronald Burton
金额：
$ 60.06万
依托单位：
University of California-San Diego Scripps Inst of Oceanography
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2016
资助国家：
美国
起止时间：
2016-03-01 至 2020-02-29
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1556466&HistoricalAwards=false
关键词：
Collaborative Research Molecular Basis Novel

项目摘要

Climate change affects the geographic distribution of populations and species and can result in increased opportunities for hybridization between previously isolated populations. Hybridization can have a range of outcomes. Most frequently, hybridization results in loss of fitness (hybrid breakdown), effectively reducing interbreeding and promoting the formation of new species. Occasionally, however, novel combinations of genes yield ecological advantages to hybrids, including adaptation to new ecological niches. What attributes of parental populations predict the outcome of hybridization? Answering this question is the focus of this project, and will provide new insights into the effects of climate and ecological changes on adaptation rates and species formation. The researchers also will engage in novel public informal education at the Birch Aquarium and in hands-on experiences in marine biology for 5th grade students in underserved San Diego neighborhoods. Predicting hybridization outcomes requires an understanding of the molecular basis of hybrid phenotypes. This project will conduct genomic analyses of hybrids produced by crosses between divergent populations of the copepod Tigriopus californicus. Previous work has shown that energy production by mitochondria is frequently disrupted in hybrids. Whole genome sequencing will be used to determine genetic differences between groups of hybrid copepods with normal versus disrupted mitochondrial performance; differentiation between the groups will identify candidate genes that are potentially responsible for hybrid breakdown. Results will address: a) Does hybridization result in widespread incompatibilities across the genome, or can targeted genes be identified? b) Are the same genomic regions responsible for hybrid breakdown in different interpopulation crosses? A similar approach will be used to examine the genomic basis of improved hybrid thermal tolerance. What genetic interactions allow some hybrids to survive temperatures that are lethal to both parental populations? Is hybrid performance predictable from genetic or ecological differences between parental populations?

气候变化影响种群和物种的地理分布，并可能导致以前孤立的种群之间杂交的机会增加。杂交可以产生一系列结果。最常见的是，杂交会导致适应性丧失（杂交崩溃），从而有效地减少杂交并促进新物种的形成。然而，偶尔，新的基因组合会给杂交品种带来生态优势，包括适应新的生态位。亲本群体的哪些属性可以预测杂交的结果？回答这个问题是该项目的重点，并将为气候和生态变化对适应率和物种形成的影响提供新的见解。研究人员还将在伯奇水族馆进行新颖的公共非正式教育，并为圣地亚哥服务不足的社区的五年级学生提供海洋生物学的实践经验。预测杂交结果需要了解杂交表型的分子基础。该项目将对桡足类 Tigriopus californicus 不同种群之间杂交产生的杂种进行基因组分析。先前的研究表明，杂交种中线粒体的能量产生经常受到干扰。全基因组测序将用于确定线粒体性能正常与受损的杂种桡足类群体之间的遗传差异；群体之间的差异将识别出可能导致杂交破坏的候选基因。结果将解决：a) 杂交是否会导致整个基因组广泛不相容，或者是否可以识别目标基因？ b) 不同群体间杂交中的杂交破坏是否由相同的基因组区域负责？类似的方法将用于检查改善杂种耐热性的基因组基础。哪些遗传相互作用使一些杂交品种能够在对亲本种群都致命的温度下生存？杂交性能是否可以通过亲本种群之间的遗传或生态差异来预测？

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Developmental rate displays effects of inheritance but not of sex in interpopulation hybrids of Tigriopus californicus

DOI：
10.1002/jez.2709
发表时间：
2023-05-24
期刊：
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
影响因子：
2.8
作者：
Healy，Timothy M. M.;Hargadon，Alexis Cody;Burton，Ronald S. S.
通讯作者：
Burton，Ronald S. S.

Strong selective effects of mitochondrial DNA on the nuclear genome

DOI：
10.1073/pnas.1910141117
发表时间：
2020-03-24
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Healy, Timothy M.;Burton, Ronald S.
通讯作者：
Burton, Ronald S.

Genetic incompatibilities in reciprocal hybrids between populations of Tigriopus californicus with low to moderate mitochondrial sequence divergence