Biophotonics: Next Generation of Ultrabright Nonorganic Markers for Ultrasensitive Fluorescent Biosensors

Nayfeh拟议研究的目标是开发用于荧光生物传感器的生物光子学标记，这种标记比现有的标记更小、更明亮、更不脆弱、更实用。这项研究的具体组成部分包括：(1)在不同的入射激发强度下，在UV、可见光和近红外频率范围内，检查发射、光稳定性和漂白的时间动态；(2)检查粒子在不同环境条件下的溶解性及其亮度的完整性，例如在体内遇到的情况；以及(3)开发包覆方法以提高生物相容性，(4)开发改进合成和扩大产量的方法，以满足高需求的商业应用，以及(5)研究通过将更多的分子附加到颗粒表面来修饰颗粒的可行性，以产生能够寻找特定生物靶标的“智能”颗粒，用于成像、靶向药物输送或摧毁致病入侵者。

0118053NayfehThe objective of the proposed research is to develop biophotonic markers for fluorescent biosensors that are smaller, brighter, less fragile, and more practical than existing markers. The specific components of this research include: (1) an examination of the time dynamics of emission, photostability, and bleaching under a variety of incident excitation intensities, in the UV, visible, and near-infrared range of frequencies, (2) an examination of the solubility of particles and their integrity of brightness under diverse environmental conditions, such as those encountered in vivo, and (3) the development of cladding methods to improve biocompatibility, (4) develop methods to refine synthesis and scale the throughput to meet high-demand commercial applications, and (5) examine the feasibility of modifying the particles by attaching additional molecules to their surfaces to produce "smart" particles that are able to seek out specific biological targets, for imaging, targeted drug delivery, or destruction of a pathogenic invader.