Biophotonics: Next Generation of Ultrabright Nonorganic Markers for Ultrasensitive Fluorescent Biosensors

0118053Nayfeh拟议研究的目的是开发用于荧光生物传感器的生物光子标记物，其比现有标记物更小、更亮、更不脆弱且更实用。 这项研究的具体内容包括：（1）在UV、可见光和近红外频率范围内，在各种入射激发强度下，检测发射、光稳定性和漂白的时间动力学，（2）在各种环境条件下，例如在体内遇到的环境条件下，检测颗粒的溶解度和它们的亮度完整性，和（3）开发包覆方法以改善生物相容性，（4）开发改进合成和扩大生产量以满足高需求的商业应用的方法，和（5）通过将额外的分子附着到颗粒表面以产生“智能”颗粒来检查改性颗粒的可行性，所述“智能”颗粒能够寻找特定的生物靶，用于成像，有针对性的药物输送，或破坏病原体入侵者。

0118053NayfehThe objective of the proposed research is to develop biophotonic markers for fluorescent biosensors that are smaller, brighter, less fragile, and more practical than existing markers. The specific components of this research include: (1) an examination of the time dynamics of emission, photostability, and bleaching under a variety of incident excitation intensities, in the UV, visible, and near-infrared range of frequencies, (2) an examination of the solubility of particles and their integrity of brightness under diverse environmental conditions, such as those encountered in vivo, and (3) the development of cladding methods to improve biocompatibility, (4) develop methods to refine synthesis and scale the throughput to meet high-demand commercial applications, and (5) examine the feasibility of modifying the particles by attaching additional molecules to their surfaces to produce "smart" particles that are able to seek out specific biological targets, for imaging, targeted drug delivery, or destruction of a pathogenic invader.