Biophotonics: High-Resolution Photothermal Imaging of Non-Fluorescent Living Cells

本研究的目的是开发一种具有高吸收灵敏度和高分辨率的激光光热相对比成像（LPPI）新方法，用于检测细胞和亚细胞水平的纳米尺度非荧光结构。具体的研究目标是：(1)通过使用纳米尺度脂质体作为光热（PT）探针校准LPPI，评估LPPI在时间分辨模式下的空间分辨率和灵敏度；(2)通过将时间分辨PT图像结构作为泵浦激光能量和波长的函数与常规生化数据进行比较，研究激光脉冲对LPPI可视化细胞的影响；(3)开发细胞PT光谱寿命成像和复杂纳米尺度吸收结构的PT时间分辨分析算法，并通过多色PT探针模型进行验证。

0119470ZharovThe objective of the proposed research is to develop a new method for laser photothermal phase-contrast imaging (LPPI) which has high absorption sensitivity and high resolution for sensing nano-scale non-fluorescent structures at the cellular and sub-cellular levels. The specific research objectives are to: (1) evaluate spatial resolution and sensitivity of LPPI in a time-resolved mode by calibrating LPPI by using nano-scale liposomes as photothermal (PT) probes, (2) examine the effects of a laser pulse on a cell visualized by LPPI by comparing the time-resolved PT-image structure as a function of pump laser energy and wavelength with conventional biochemical data, and (3) develop cell PT spectral lifetime imaging and algorithms of PT time-resolved analysis of complex nano-scale absorbing structures verified with a multi-color PT probe model.