The Cnidarian Genetic Toolkit

刺胞动物遗传工具包

• 批准号: 0120591
• 负责人: Hans Bode
• 金额: --
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-10-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0120591&HistoricalAwards=false
• 关键词: Cnidarian; Genetic; Toolkit

0120591BodeIt is becoming increasingly clear that understanding how an organism interacts with its environment will require a complete accounting of the genes which make up the organism and the expression patterns of the genes under various conditions. Members of the phylum Cnidaria (jellyfish, corals, sea anemones, and hydras) are key members of their environments, yet very little is known about their genetic makeup. To address this problem, a collection of cloned DNAs representing the messenger RNA populations (cDNAs) of two well-studied cnidarians will be generated. The two organisms that will be used are the freshwater cnidarian Hydra and the colonial marine cnidarian Podocoryne. Bacteria containing the cloned cDNAs will be robotically arrayed to generate archives of the clones. Sequence data will be obtained from approximately 50,000 of the cDNA clones from each organism. The resulting sequence data will be analyzed in various ways using bioinformatic computing tools. The analyses will provide information on what genes are present in cnidarians and how those genes are evolutionarily related to those in animals which diverged more recently than cnidarians, such as vertebrates and insects. Such information will be very valuable for defining the processes by which multicellular animals have evolved. The availability of cloned cDNA sets from two model cnidarians will also make it possible to examine the expression of large numbers of genes in these organisms using the technique of DNA array analysis. In particular it will be possible to identify genes whose expression changes when the organisms are placed under conditions which reflect those present during periods of environmental stress (e.g. elevated temperature). Genes whose expression levels change under stress conditions in the laboratory may be useful tools for monitoring the health of cnidarians (e.g. corals) in their natural setting.

0120591Bode越来越清楚的是，了解有机体如何与其环境相互作用将需要对构成有机体的基因以及这些基因在各种条件下的表达模式进行完整的解释。刺胞动物门的成员（水母、珊瑚、海葵和水螅）是其环境的关键成员，但人们对其基因组成知之甚少。为了解决这个问题，将生成代表两种经过充分研究的刺胞动物的信使 RNA 群体 (cDNA) 的克隆 DNA 集合。将使用的两种生物是淡水刺胞动物九头蛇和殖民海洋刺胞动物Podocoryne。含有克隆 cDNA 的细菌将被自动排列以生成克隆档案。序列数据将从每个生物体的大约 50,000 个 cDNA 克隆中获得。由此产生的序列数据将使用生物信息学计算工具以各种方式进行分析。这些分析将提供有关刺胞动物中存在哪些基因以及这些基因如何在进化上与比刺胞动物分化更晚的动物（例如脊椎动物和昆虫）中的基因相关的信息。这些信息对于定义多细胞动物的进化过程非常有价值。来自两种模型刺胞动物的克隆 cDNA 组的可用性也使得使用 DNA 阵列分析技术检查这些生物体中大量基因的表达成为可能。特别是，当生物体置于反映环境应激期间（例如升高的温度）期间存在的条件时，将有可能鉴定其表达发生变化的基因。在实验室压力条件下表达水平发生变化的基因可能是监测自然环境中刺胞动物（例如珊瑚）健康状况的有用工具。