Ligand Translational Mobility and Concentration Near Membranes
配体的平移迁移率和膜附近的浓度
基本信息
- 批准号:0130589
- 负责人:
- 金额:$ 37.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-03-01 至 2007-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The hypothesis of this project is that local deviations from ideal, bulk translational mobilities and concentrations of ligands near membrane surfaces exert critical influences on the kinetics of ligand-receptor interactions and, as a consequence, biological function. To test this hypothesis, three groups of specific aims will be pursued. In the first group of aims, physical factors (electrostatic, hydrodynamic and entrapment) governing the translational mobilities and concentrations of fluorescent ligands in regions of solution that are very close to model and natural cell membranes deposited on transparent planar substrates will be examined. These measurements will be carried out by using total internal reflection with fluorescence correlation spectroscopy as developed during the last funding period. In the second group of specific aims, three technological innovations will be implemented. These innovations will provide distance-dependent, rather than distance-averaged, information about local ligand dynamics. First, the distance-dependence of the local ligand translational mobility and concentration will be probed by changing the evanescent depth. Second, photon counting histograms obtained with evanescent illumination will provide a direct measure of the manner in which the ligand concentration and therefore potential depend on the distance from the membrane surface. Third, high order autocorrelation will be employed to further confirm and understand the effects of the nearby membrane surface on the local ligand concentration and mobility. The goal of the final specific aim is to correlate local changes in ligand concentrations and dynamics, as they are observed, with specific ligand-receptor kinetics. The interaction kinetics will be monitored with a well-established method, total internal reflection with fluorescence photobleaching recovery.
该项目的假设是,与理想的局部偏差,膜表面附近的大块翻译迁移率和配体浓度对配体-受体相互作用的动力学产生关键影响,从而影响生物功能。为了验证这一假设,将追求三组具体目标。在第一组目标中,将研究控制荧光配体在非常接近模型和沉积在透明平面基底上的天然细胞膜的溶液区域中的平移迁移率和浓度的物理因素(静电、水动力和捕获)。这些测量将使用在上一个资助期间开发的全内反射荧光相关光谱学进行。在第二组具体目标中,将实施三项技术创新。这些创新将提供有关局部配体动力学的距离依赖信息,而不是距离平均信息。首先,通过改变消失深度来探讨局部配体平移迁移率和浓度的距离依赖性。其次,用瞬时照明获得的光子计数直方图将提供一种直接测量配体浓度的方式,因此电位取决于与膜表面的距离。第三,利用高阶自相关进一步确认和了解邻近膜表面对局部配体浓度和迁移率的影响。最终具体目标的目标是将观察到的配体浓度和动力学的局部变化与特定配体-受体动力学联系起来。相互作用动力学将监测与完善的方法,全内反射与荧光光漂白回收。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Nancy Thompson其他文献
Women's imprisonment : a study in social control
妇女监禁:社会控制研究
- DOI:
- 发表时间:
1984 - 期刊:
- 影响因子:0
- 作者:
Nancy Thompson;P. Carlen - 通讯作者:
P. Carlen
20-P007 Role of Neurogenin3 in the migration of pancreatic endocrine cells
- DOI:
10.1016/j.mod.2009.06.840 - 发表时间:
2009-08-01 - 期刊:
- 影响因子:
- 作者:
Mathieu Gouzi;Kerstin Johansson;Nancy Thompson;Josselin Soyer;Gérard Gradwohl;Anne Grapin-Botton - 通讯作者:
Anne Grapin-Botton
Under-replication of intron+ rDNA cistrons in polyploid nurse cell nuclei of Calliphora erythrocephala
- DOI:
10.1007/bf00338488 - 发表时间:
1982-12-01 - 期刊:
- 影响因子:2.300
- 作者:
Kathy Beckingham;Nancy Thompson - 通讯作者:
Nancy Thompson
A5019 - Laparoscopic Gastric Plication in Pediatric Patients with Morbid Obesity
- DOI:
10.1016/j.soard.2017.09.165 - 发表时间:
2017-10-01 - 期刊:
- 影响因子:
- 作者:
Jonathan DeAntonio;Hannah Cockrell;Hae Sung Kang;Nancy Thompson;Claudio Oiticica;David Lanning - 通讯作者:
David Lanning
Nancy Thompson的其他文献
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{{ truncateString('Nancy Thompson', 18)}}的其他基金
FSML: Improvements to the Keys Marine Laboratory Seawater System
FSML:Keys 海洋实验室海水系统的改进
- 批准号:
1319005 - 财政年份:2013
- 资助金额:
$ 37.5万 - 项目类别:
Standard Grant
Biological Reaction Networks Characterized by Total Internal Reflection with Fluorescence Correlation Spectroscopy
荧光相关光谱全内反射表征的生物反应网络
- 批准号:
0641087 - 财政年份:2007
- 资助金额:
$ 37.5万 - 项目类别:
Continuing Grant
Protein Translational Mobility and Concentration Near Membrane Surfaces
膜表面附近的蛋白质翻译迁移率和浓度
- 批准号:
9728116 - 财政年份:1998
- 资助金额:
$ 37.5万 - 项目类别:
Continuing Grant
Faculty Award for Women Scientists and Engineers
女科学家和工程师学院奖
- 批准号:
9024028 - 财政年份:1991
- 资助金额:
$ 37.5万 - 项目类别:
Continuing Grant
Presidential Young Investigator Award
总统青年研究员奖
- 批准号:
8552986 - 财政年份:1986
- 资助金额:
$ 37.5万 - 项目类别:
Continuing Grant
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