U.S.-Czech Republic Research on Microarray-Based Analysis of Plant Genome Structure.

亚利桑那大学的大卫·加尔布雷斯和捷克科学院奥洛穆克实验植物学研究所的雅罗斯拉夫·多莱泽尔共同开展了这项由美国和捷克联合开展的研究项目，其特点是利用花分类选择性地丰富大麦个体染色体的群体。他们的目的是生产用于大麦DNA微阵列杂交和中期染色体荧光原位杂交(FISH)分析的荧光基因组DNA靶标。总的努力得益于亚利桑那州团队在微阵列技术方面的广泛专业知识，以及捷克在植物染色体荧光激活分类和植物染色体细胞学方面的专业知识。本科生的研究经验是研究计划不可或缺的一部分。如果成功，这项工作将产生以大麦为模型在特定植物染色体上定位单拷贝基因(即EST)的新技术。研究人员还预计将使用已分类的染色体来生产特定于染色体的DNA文库。这一植物基因组研究的国际项目通过使美国和中欧的专家能够结合互补的人才并在相互感兴趣和能力很强的领域共享研究资源，实现了促进科学知识的计划目标。

This U.S.-Czech research project between David Galbraith of the University of Arizona and his counterpart, Jaroslav Dolezel from the Czech Academy of Sciences' Institute of Experimental Botany in Olomouc, features use of flowsorting to selectively enrich populations of individual barley chromosomes. Their intent is to produce fluorescent genomic DNA targets for hybridization of barley DNA microarrays and for fluorescent in situ hybridization (FISH) analyses of metaphase chromosomes. The total effort benefits from the Arizona team's extensive expertise with microarray technologies and the Czech's expertise in both fluorescence-activated sorting of plant chromosomes and plant chromosome cytology. Research experiences for undergraduate students are an integral part of the research plan. If successful, this work my yield new techniques for mapping single copy genes (i.e. ESTs) on specific plant chromosomes using barley as a model. The researchers also anticipate using the sorted chromosomes for the production of chromosome-specific DNA libraries. This international project in plant genome research fulfills the program objective of advancing scientific knowledge by enabling experts in the Untied States and Central Europe to combine complementary talents and share research resources in areas of strong mutual interest and competence.