U.S.-Czech Republic Research on Microarray-Based Analysis of Plant Genome Structure.

这项由亚利桑那大学的David Galbraith和捷克科学院位于奥洛穆茨的实验植物学研究所的Jaroslav Dolezel共同开展的美国-捷克研究项目，利用流动分选技术选择性地丰富单个大麦染色体的种群。他们的目的是为大麦DNA微阵列杂交和中期染色体荧光原位杂交（FISH）分析生产荧光基因组DNA靶标。总的努力得益于亚利桑那团队在微阵列技术方面的广泛专业知识，以及捷克团队在植物染色体荧光激活分选和植物染色体细胞学方面的专业知识。本科生的研究经历是研究计划的一个组成部分。如果成功，这项工作将产生以大麦为模型在特定植物染色体上绘制单拷贝基因（即ESTs）的新技术。研究人员还期望使用分类的染色体来生产染色体特异性DNA文库。这个国际植物基因组研究项目通过使美国和中欧的专家能够在共同感兴趣和能力强的领域结合互补的人才和共享研究资源，实现了推进科学知识的计划目标。

This U.S.-Czech research project between David Galbraith of the University of Arizona and his counterpart, Jaroslav Dolezel from the Czech Academy of Sciences' Institute of Experimental Botany in Olomouc, features use of flowsorting to selectively enrich populations of individual barley chromosomes. Their intent is to produce fluorescent genomic DNA targets for hybridization of barley DNA microarrays and for fluorescent in situ hybridization (FISH) analyses of metaphase chromosomes. The total effort benefits from the Arizona team's extensive expertise with microarray technologies and the Czech's expertise in both fluorescence-activated sorting of plant chromosomes and plant chromosome cytology. Research experiences for undergraduate students are an integral part of the research plan. If successful, this work my yield new techniques for mapping single copy genes (i.e. ESTs) on specific plant chromosomes using barley as a model. The researchers also anticipate using the sorted chromosomes for the production of chromosome-specific DNA libraries. This international project in plant genome research fulfills the program objective of advancing scientific knowledge by enabling experts in the Untied States and Central Europe to combine complementary talents and share research resources in areas of strong mutual interest and competence.