U.S.-Czech Republic Research on Microarray-Based Analysis of Plant Genome Structure.

美国-捷克共和国基于微阵列的植物基因组结构分析研究。

• 批准号: 0130671
• 负责人: David Galbraith
• 金额: $ 4.04万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0130671&HistoricalAwards=false
• 关键词: U.S.; Czech; Republic; Research; Microarray

This U.S.-Czech research project between David Galbraith of the University of Arizona and his counterpart, Jaroslav Dolezel from the Czech Academy of Sciences' Institute of Experimental Botany in Olomouc, features use of flowsorting to selectively enrich populations of individual barley chromosomes. Their intent is to produce fluorescent genomic DNA targets for hybridization of barley DNA microarrays and for fluorescent in situ hybridization (FISH) analyses of metaphase chromosomes. The total effort benefits from the Arizona team's extensive expertise with microarray technologies and the Czech's expertise in both fluorescence-activated sorting of plant chromosomes and plant chromosome cytology. Research experiences for undergraduate students are an integral part of the research plan. If successful, this work my yield new techniques for mapping single copy genes (i.e. ESTs) on specific plant chromosomes using barley as a model. The researchers also anticipate using the sorted chromosomes for the production of chromosome-specific DNA libraries. This international project in plant genome research fulfills the program objective of advancing scientific knowledge by enabling experts in the Untied States and Central Europe to combine complementary talents and share research resources in areas of strong mutual interest and competence.

亚利桑那大学的戴维·加尔布雷思（David Galbraith）与他的对手捷克斯拉夫·多赛尔（Jaroslav Dolezel）的美国研究项目，来自捷克大学科学院实验植物学研究所的jaroslav dolezel在Olomouc的实验性植物学研究所，具有量供应有选择性地富含单个大脂肪体的人群。他们的目的是产生荧光基因组DNA靶标，以杂交大麦DNA微阵列和中期染色体的原位杂交（FISH）分析。总体上的努力受益于亚利桑那团队在微阵列技术方面的广泛专业知识以及捷克的植物染色体和植物染色体细胞学的荧光激活分类方面的专业知识。本科生的研究经验是研究计划不可或缺的一部分。 如果成功，这项工作我的产生了新技术，用于在特定的植物染色体上使用大麦作为模型映射单复制基因（即EST）。研究人员还预计，使用分类的染色体来生产染色体特异性DNA文库。这项国际植物基因组研究项目实现了计划的目标，即通过使无纽带和中欧的专家能够在浓厚的共同利益和能力的领域中共享互补才能并共享研究资源，从而实现了促进科学知识的计划。