Acquisition of Equipment for High-throughput Genomics Studies

购置高通量基因组学研究设备

• 批准号: 0070349
• 负责人: David Galbraith
• 金额: $ 31.9万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2003-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0070349&HistoricalAwards=false
• 关键词: Acquisition; Equipment; throughput; Genomics; Studies

Abstract Galbraith During the last few years, the analysis of entire genomes has been made possible by technological breakthroughs, such that gene isolation and characterization and functional analyses have become dramatically faster and more efficient than previously possible. "Genomics" is broadly defined as research toward understanding the structure of genomes, the genes present in these genomes and their expression, and the functions of the proteins encoded by these genes. Essential to genome-wide gene expression and function studies is the use of recently developed robotic methods for the high-throughput manipulation of genes and the analysis of sequences. Equipment for colony and plaque picking (a robotic workstation), for DNA sequence amplification (PCR instruments), for high-throughput DNA sequencing (a capillary DNA sequencer), and for analysis of DNA microarrays (a microarray scanner) will be used for high-throughput genomics analysis at the University of Arizona. The colony and plaque-picking robot will be used for the high-throughput isolation of individual colonies and plaques from cDNA, EST, and genomic libraries produced for different eukaryotic and prokaryotic organisms and tissues. cDNA/EST libraries will be produced under a variety of conditions designed to induce different expression patterns, thereby identifying new genes. The PCR amplification instruments will be employed for the high-throughput production of amplified DNA molecules suitable for DNA sequencing and DNA microarraying. The capillary sequencer will be employed for the sequencing of the DNA molecules that have been amplified from the various library inserts. The array scanner will be employed for the analysis of microarrays following hybridization using fluorescent "target" molecules prepared from eukaryotic cells and tissues subjected to different environmental conditions. The impact of the instrumentation on the field of research will be particularly profound in the area of the Plant Sciences. Four major NSF-funded Plant Genomics grants provide research funding for the University of Arizona. Given the availability of high-throughput instrumentation provided by this funding, a considerable increase in the progress of these projects is anticipated. Specifically, the instrumentation will permit the production and sequencing of many more cDNA and EST libraries than originally envisaged by these proposals. This should lead to greater numbers of discovered genes for different organisms, as well as a more profound understanding of how gene expression is regulated. The instrumentation will also alleviate bottlenecks in PCR-based microarray production and microarray analysis, and this in turn will accelerate understanding of the coordination of gene expression through functional genomics. These research and training groups represent ones at the University of Arizona that are already involved in genomics analyses. Other U.A. groups are poised to start such work as a logical extension of their ongoing, federally funded activities. Availability of the proposed equipment will directly assist their programs, and also increase the numbers of faculty groups considering a high-throughput genomics approach to their specific research problems. In terms of education and outreach, the U.A. has a defined mission in educating the future work force of Arizona and the Nation. The availability of the proposed equipment will provide an invaluable teaching and training resource.

摘要Galbraith在过去几年中，通过技术突破使整个基因组的分析成为可能，从而使基因隔离，表征和功能分析变得比以前更快，更有效。 “基因组学”广泛定义为研究基因组的结构，这些基因组中存在的基因及其表达以及这些基因编码的蛋白质的功能。全基因组基因表达和功能研究必不可少的是使用最近开发的机器人方法来进行基因的高通量操纵和序列分析。用于殖民地和斑块采摘的设备（机器人工作程序），用于DNA序列扩增（PCR仪器），用于高通量DNA测序（毛细管DNA Sequencer）的设备，以及用于在Ariz of Arizone of Arizone of Arizons分析的高通用基因组分析的DNA微阵列（微阵列扫描仪）。菌落和斑块采摘机器人将用于从cDNA，EST和基因组库中的单个菌落和斑块的高通量分离，用于不同的真核生物和原核生物体和组织。 将在旨在诱导不同表达模式的各种条件下生产cDNA/EST文库，从而识别新基因。 PCR扩增仪器将用于高通量产生适合DNA测序和DNA微阵列的放大DNA分子。 毛细血管序列将用于从各种库插入物中放大的DNA分子的测序。 阵列扫描仪将使用荧光“靶”分子在经受不同环境条件下制备的荧光“靶”分子进行杂交后进行微阵列分析。 在植物科学领域，仪器对研究领域的影响将特别深刻。 NSF资助的四项主要植物基因组学赠款为亚利桑那大学提供了研究资金。鉴于该资金提供的高通量仪器的可用性，预计这些项目的进度将大大增加。 具体而言，该仪器将允许与这些建议最初设想的更多cDNA和EST库的生产和测序。这应该导致更多的不同生物的发现基因，以及对如何调节基因表达的更深入的理解。该仪器还将减轻基于PCR的微阵列生产和微阵列分析中的瓶颈，这反过来又将加速通过功能基因组学对基因表达的协调的理解。 这些研究和培训小组代表亚利桑那大学的研究小组已经参与了基因组学分析。美国其他团体准备开始进行此类工作，例如对正在进行的联邦资助活动的逻辑扩展。拟议设备的可用性将直接协助他们的计划，并增加考虑针对其特定研究问题的高通量基因组学方法的教师小组的数量。在教育和宣传方面，英国在教育亚利桑那州和国家的未来劳动力方面具有明确的使命。拟议设备的可用性将提供宝贵的教学和培训资源。