Acquisition of Equipment for High-throughput Genomics Studies

购置高通量基因组学研究设备

• 批准号: 0070349
• 负责人: David Galbraith
• 金额: $ 31.9万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2003-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0070349&HistoricalAwards=false
• 关键词: Acquisition; Equipment; throughput; Genomics; Studies

Abstract Galbraith During the last few years, the analysis of entire genomes has been made possible by technological breakthroughs, such that gene isolation and characterization and functional analyses have become dramatically faster and more efficient than previously possible. "Genomics" is broadly defined as research toward understanding the structure of genomes, the genes present in these genomes and their expression, and the functions of the proteins encoded by these genes. Essential to genome-wide gene expression and function studies is the use of recently developed robotic methods for the high-throughput manipulation of genes and the analysis of sequences. Equipment for colony and plaque picking (a robotic workstation), for DNA sequence amplification (PCR instruments), for high-throughput DNA sequencing (a capillary DNA sequencer), and for analysis of DNA microarrays (a microarray scanner) will be used for high-throughput genomics analysis at the University of Arizona. The colony and plaque-picking robot will be used for the high-throughput isolation of individual colonies and plaques from cDNA, EST, and genomic libraries produced for different eukaryotic and prokaryotic organisms and tissues. cDNA/EST libraries will be produced under a variety of conditions designed to induce different expression patterns, thereby identifying new genes. The PCR amplification instruments will be employed for the high-throughput production of amplified DNA molecules suitable for DNA sequencing and DNA microarraying. The capillary sequencer will be employed for the sequencing of the DNA molecules that have been amplified from the various library inserts. The array scanner will be employed for the analysis of microarrays following hybridization using fluorescent "target" molecules prepared from eukaryotic cells and tissues subjected to different environmental conditions. The impact of the instrumentation on the field of research will be particularly profound in the area of the Plant Sciences. Four major NSF-funded Plant Genomics grants provide research funding for the University of Arizona. Given the availability of high-throughput instrumentation provided by this funding, a considerable increase in the progress of these projects is anticipated. Specifically, the instrumentation will permit the production and sequencing of many more cDNA and EST libraries than originally envisaged by these proposals. This should lead to greater numbers of discovered genes for different organisms, as well as a more profound understanding of how gene expression is regulated. The instrumentation will also alleviate bottlenecks in PCR-based microarray production and microarray analysis, and this in turn will accelerate understanding of the coordination of gene expression through functional genomics. These research and training groups represent ones at the University of Arizona that are already involved in genomics analyses. Other U.A. groups are poised to start such work as a logical extension of their ongoing, federally funded activities. Availability of the proposed equipment will directly assist their programs, and also increase the numbers of faculty groups considering a high-throughput genomics approach to their specific research problems. In terms of education and outreach, the U.A. has a defined mission in educating the future work force of Arizona and the Nation. The availability of the proposed equipment will provide an invaluable teaching and training resource.

在过去的几年里，整个基因组的分析已经成为可能的技术突破，这样的基因分离和表征和功能分析已经变得显着更快，更有效地比以前可能的。“基因组学”被广泛定义为研究基因组的结构，存在于这些基因组中的基因及其表达，以及这些基因编码的蛋白质的功能。全基因组基因表达和功能研究的关键是使用最近开发的机器人方法进行基因的高通量操作和序列分析。用于菌落和噬菌斑挑选（机器人工作站），DNA序列扩增（PCR仪器），高通量DNA测序（毛细管DNA测序仪）和DNA微阵列分析（微阵列扫描仪）的设备将用于亚利桑那大学的高通量基因组学分析。殖民地和噬菌斑拾取机器人将用于从为不同真核和原核生物和组织产生的cDNA、EST和基因组文库中高通量分离单个菌落和噬菌斑。 cDNA/EST文库将在设计用于诱导不同表达模式的各种条件下产生，从而鉴定新基因。 PCR扩增仪器将用于高通量生产适合DNA测序和DNA微阵列的扩增DNA分子。 毛细管测序仪将用于对从各种文库插入物扩增的DNA分子进行测序。 阵列扫描仪将用于分析杂交后的微阵列，使用荧光“靶”分子，从真核细胞和组织中制备，经受不同的环境条件。 仪器对研究领域的影响将在植物科学领域特别深刻。 四个主要的NSF资助的植物基因组学赠款为亚利桑那大学提供研究资金。鉴于这笔资金提供了高通量仪器，预计这些项目的进展将大大增加。 具体地说，仪器将允许产生和测序比这些建议最初设想的更多的cDNA和EST文库。这应该会导致更多的不同生物体的基因被发现，以及对基因表达是如何调节的更深刻的理解。该仪器还将缓解基于PCR的微阵列生产和微阵列分析中的瓶颈，这反过来将通过功能基因组学加速对基因表达协调的理解。 这些研究和培训小组代表了亚利桑那大学已经参与基因组学分析的小组。其他美国各团体准备开始这项工作，作为其正在进行的、由联邦政府资助的活动的合理延伸。拟议设备的可用性将直接帮助他们的计划，并增加考虑高通量基因组学方法来解决其特定研究问题的教师群体的数量。在教育和推广方面，有一个明确的使命在教育亚利桑那州和国家的未来劳动力。拟议设备的提供将提供宝贵的教学和培训资源。