Flow Cytometry: Digital Processing of Molecular Information

该奖项提供了用于分析大细胞(直径10至50微米)，主要是植物细胞原生质体的流式细胞仪技术和仪器的开发。重点将是开发用于实时分析和分类的数字脉冲处理模块。还将探索用于转基因植物的新型底物和基因表达系统的开发。最后，这些技术将被用于选择表达发育调节基因的转基因植物细胞群体，作为构建组织特异性DNA文库的第一步。

This award provides for the development of flow cytometric techniques and instrumentation for the analysis of large cell (10 to 50 microns in diameter), principally plant cell protoplasts. The emphasis will be on development of digital pulse processing modules for real time analysis and sorting. The development of novel substrates and gene expression systems for use with transgenic plants will also be explored. Finally, these techniques will be used to select populations of transgenic plant cells expressing developmentally regulated genes as a first step in the construction of tissue specific-DNA libraries.