Flow Cytometry: Digital Processing of Molecular Information

该奖项旨在发展流式细胞术， 分析大细胞的技术和仪器（10 至50微米直径），主要是植物细胞原生质体。 重点是数字脉冲处理的发展 用于真实的时间分析和排序的模块。 的发展 新的底物和基因表达系统 还将研究转基因植物。 最后，这些技术 将用于选择转基因植物细胞群体 表达发育调节基因作为第一步， 组织特异性DNA文库的构建。

This award provides for the development of flow cytometric techniques and instrumentation for the analysis of large cell (10 to 50 microns in diameter), principally plant cell protoplasts. The emphasis will be on development of digital pulse processing modules for real time analysis and sorting. The development of novel substrates and gene expression systems for use with transgenic plants will also be explored. Finally, these techniques will be used to select populations of transgenic plant cells expressing developmentally regulated genes as a first step in the construction of tissue specific-DNA libraries.