Plastic Microfluidics-Based 2-D PAGE

基于塑料微流体的二维 PAGE

• 批准号: 0138007
• 负责人: Cheng Lee
• 金额: $ 32.29万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-15 至 2005-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0138007&HistoricalAwards=false
• 关键词: Plastic; Microfluidics; Based; PAGE

This award supports an effort to develop a miniaturized and integrated two-dimensional (2-D) protein separation platform based on non-native isoelectric focusing and SDS gel electrophoresis in a microfluidic network. The network will be embedded in a small plastic chip, and the device will employ laser-induced fluorescence for the detection of resolved SDS-protein complexes in the microchannel array using non-covalent, environment-sensitive, fluorescent probes. The resulting instrumentation system will be capable of analyzing complex mixtures of proteins, including crude cell lysates containing minute quantities of protein. In addition, the device will be amenable to much greater automation, throughput, and sensitivity than existing gel-based systems. This microfluidics-based instrumentation technology, positioned as the next generation of 2-D poly acrylimide gel electrophoresis, promises to have a major impact on proteomics research, particularly in comparisons of protein expression in organisms growing under different conditions.

该奖项支持在微流体网络中开发基于非天然等电聚焦和SDS凝胶电泳的小型化和集成二维（2-D）蛋白质分离平台的努力。该网络将嵌入一个小的塑料芯片中，该设备将采用激光诱导荧光，使用非共价的、环境敏感的荧光探针检测微通道阵列中的已分辨SDS-蛋白质复合物。所得到的仪器系统将能够分析复杂的蛋白质混合物，包括含有微量蛋白质的粗细胞裂解物。 此外，该设备将比现有的基于凝胶的系统具有更高的自动化、吞吐量和灵敏度。这种基于微流体的仪器技术被定位为下一代2-D聚丙烯酰胺凝胶电泳，有望对蛋白质组学研究产生重大影响，特别是在不同条件下生长的生物体中蛋白质表达的比较方面。