Instrumentation Development of Enhanced Capillary Zone Electrophoresis for Bioanalyses

用于生物分析的增强毛细管区带电泳仪器的开发

• 批准号: 9105016
• 负责人: Cheng Lee
• 金额: $ 11.45万
• 依托单位: University of Maryland Baltimore County
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1994-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105016&HistoricalAwards=false
• 关键词: Instrumentation; Development; Enhanced; Capillary; Zone

Capillary Zone Electrophoresis (CZE) is probably the simplest and perhaps the most universally useful mode of High Performance Capillary Electrophoresis (HPCE), which combines the strengths of both conventional electrophoresis in resolving power and High Performance Liquid Chromatography (HPLC) in the ease and speed of analysis. A fundamental problem in CZE is to directly control the zeta potential at the aqueous/capillary interface. Director control of the zeta potential is essential for manipulating the electroosmotic flow inside the capillary and the adsorption of macromolecules onto the capillary wall. An instrumentation method involving the use of an additional electric field from outside the capillary for controlling the zeta potential is therefore proposed in this study. The fundamentals of this approach will be studied both experimentally and theoretically with the proposed capacitor model. Significant improvements of separation efficiency and resolution of protein mixtures and DNA fragments for both qualitative and quantitative applications will then be demonstrated and achieved.

毛细管区带电泳(CZE)可能是高效毛细管电泳法(HPCE)中最简单、也是最普遍使用的模式，它结合了传统电泳法在分辨率方面的优势和高效液相色谱(HPLC)在分析简便性和速度方面的优势。CZE的一个基本问题是直接控制水/毛细管界面的Zeta电位。Zeta电位的直接控制对于控制毛细管内的电渗流和大分子在毛细管壁上的吸附是必不可少的。因此，本研究提出了一种利用毛细管外的附加电场来控制Zeta电位的仪器方法。这种方法的基本原理将从实验和理论上结合所提出的电容模型进行研究。然后，蛋白质混合物和DNA片段在定性和定量应用方面的分离效率和分辨率将得到显着改善。