Mechanistic Studies of Electroosmotic Control in Capillary Zone Electrophoresis

毛细管区带电泳电渗控制机理研究

• 批准号: 9318694
• 负责人: Cheng Lee
• 金额: $ 23.7万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9318694&HistoricalAwards=false
• 关键词: Mechanistic; Studies; Electroosmotic; Control; Capillary

CTS-9318694 Iowa State University Cheng S. Lee ABSTRACT The aim of this project is to understand certain factors in capillary zone electrophoresis (CZE) in order to develop gradient elution High Performance Capillary Electrophoresis. The magnitude and polarity of the SYMBOL 122 \f "Symbol" potential strongly influences protein adsorption as well as the direction and magnitude of electroosmotic flow. For this project, a radial potential gradient will be tested as a means of varying the SYMBOL 122 \f "Symbol" potential and thereby reducing protein adsorption and enhancing the revolving power of the technique. Dispersion studies under a hydrostatic pressure-driven flow component will be conducted in a set of tests in which the fractional length of the capillary under electroosmotic control will be changed. A series capacitance model combined Gouy-Chapman-Stern-Grahame theory will be evaluated with experimental results. CZE is a very promising technique that offers the analytical capabilities of electrophoresis and the convenience of HPLC. Its development will provide a powerful tool in support of the biotechnology and pharmaceutical industries. IBMBIO COM' ` IBMDOS COM' ` AUTOEXECBAK s" Õ FLW K PSFONTS K ! 89DECS PRE .N T" AUTOEXECBAT E J BURKA PRE dP #zs SAVERS j " ICONS j " LM Y CTS-9318694 Iowa State University Cheng S. Lee ABSTRACT The aim of this project is to understand certa in factors in capi v w 3 5 ! !J ! F ( Times New Roman Symbol & Arial , 9 9 " h #c %#c % 5 1 Nichelle Coward Nichelle Coward

爱荷华州州立大学Cheng S.李 摘要 本计画的目的是了解毛细管区带电泳（CZE）中的某些因素，以发展梯度洗脱高效毛细管电泳。 SYMBOL 122 \f“Symbol”电位的大小和极性强烈影响蛋白质吸附以及电渗流的方向和大小。 对于本项目，将测试径向电位梯度，作为改变SYMBOL 122 \f“Symbol”电位的一种手段，从而减少蛋白质吸附并提高技术的旋转能力。 将在一组测试中进行流体静压驱动流动组件下的分散研究，其中将改变电渗控制下毛细管的分数长度。 一个串联电容模型结合Gouy-Chapman-Stern-Grahame理论将与实验结果进行评估。 毛细管区带电泳是一种很有前途的技术，它提供了电泳的分析能力和HPLC的方便。 它的发展将为支持生物技术和制药工业提供一个强有力的工具。 IBM公司 ` IBMDOS COM ` 自动执行 s” FLW K PSFONTS K！ 89DECS PRE .N T” 自动执行 E J 布尔卡 预 dP #zs SAVERS J " 图标 J " LM Y 爱荷华州州立大学Cheng S.李 摘要 本课题的目的是了解CAPI中的某些因素 v W 3 5 ! ! J ! F ( Times New Roman 符号Arial , 9 9 " H #c %#c % 5 1 尼歇尔·科沃德尼歇尔·科沃德