Mechanistic Studies of Electroosmotic Control in Capillary Zone Electrophoresis

9318694爱荷华州立大学李成摘要本项目的目的是了解毛细管区带电泳中的某些因素，以便发展梯度洗脱高效毛细管电泳法。符号122“符号”电位的大小和极性强烈地影响着蛋白质的吸附以及电渗流的方向和大小。在这个项目中，将测试径向电位梯度，作为改变符号122“符号”电位的一种手段，从而减少蛋白质吸附并增强该技术的旋转力。流体静压驱动流动组件下的弥散研究将在一组试验中进行，在该试验中，电渗透控制下的毛细管的分数长度将发生变化。结合古伊-查普曼-斯特恩-格雷厄姆理论的串联电容模型将用实验结果进行评估。毛细管电泳法是一种非常有前景的技术，它提供了电泳法的分析能力和高效液相色谱法的便利性。它的发展将为支持生物技术和制药业提供强有力的工具。IBMBIO com‘`IBMDOS com’`AUTOEXECBAK S“？FLW K PSFONTS K！89DECS PRE.N”AUTOEXECBAT E J Burka PRE DP#zs saver j“图标j“LYCTS-9318694爱荷华州立大学李成山摘要本项目的目的是了解CAPI与W因素中的CETA。3 5！！J！F(Times New Roman符号&amp；Arial，9 9“h#c%#c%5 1 Nichelle Coward Nichelle Coward

CTS-9318694 Iowa State University Cheng S. Lee ABSTRACT The aim of this project is to understand certain factors in capillary zone electrophoresis (CZE) in order to develop gradient elution High Performance Capillary Electrophoresis. The magnitude and polarity of the SYMBOL 122 \f "Symbol" potential strongly influences protein adsorption as well as the direction and magnitude of electroosmotic flow. For this project, a radial potential gradient will be tested as a means of varying the SYMBOL 122 \f "Symbol" potential and thereby reducing protein adsorption and enhancing the revolving power of the technique. Dispersion studies under a hydrostatic pressure-driven flow component will be conducted in a set of tests in which the fractional length of the capillary under electroosmotic control will be changed. A series capacitance model combined Gouy-Chapman-Stern-Grahame theory will be evaluated with experimental results. CZE is a very promising technique that offers the analytical capabilities of electrophoresis and the convenience of HPLC. Its development will provide a powerful tool in support of the biotechnology and pharmaceutical industries. IBMBIO COM' ` IBMDOS COM' ` AUTOEXECBAK s" Õ FLW K PSFONTS K ! 89DECS PRE .N T" AUTOEXECBAT E J BURKA PRE dP #zs SAVERS j " ICONS j " LM Y CTS-9318694 Iowa State University Cheng S. Lee ABSTRACT The aim of this project is to understand certa in factors in capi v w 3 5 ! !J ! F ( Times New Roman Symbol & Arial , 9 9 " h #c %#c % 5 1 Nichelle Coward Nichelle Coward