RUI: Polyphasic Characterization and Phylogenetic Classification of the Problematic Form-Genus Leptolyngbya (Cyanobacteria)

RUI：有问题的形式-Leptolyngbya（蓝细菌）属的多相特征和系统发育分类

• 批准号: 0206360
• 负责人: Jeffrey Johansen
• 金额: $ 15万
• 依托单位: John Carroll University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2005-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0206360&HistoricalAwards=false
• 关键词: RUI; Polyphasic; Characterization; Phylogenetic; Classification

A grant has been awarded to Dr. Jeffrey R. Johansen of John Carroll University to study a problematic genus of blue-green algae (cyanobacteria) called Leptolyngbya. This genus is difficult to understand because it has very few morphological characters, but occurs in a wide variety of habitat types. Morphologically similar forms have been found to be actually very different in terms of their 16S rRNA gene sequence. Dr. Johansen will use a polyphasic approach to characterize over 60 strains of this microbe from collections all over the world, including an extensive collection of desert soil forms isolated as part of a past NSF-supported project. Polyphasic characterization means that morphological, genetic, ecological, and physiological data will be collected on these strains, so that the genus can be revised. Preliminary evidence indicates that there are many new species in the genus, and probably even groups of species that merit creation of new genera. Unlike all other cyanobacterial taxonomic studies and most bacteriological systematic studies, this project will test the applicability of three modern species concepts for asexual species. Further precedents will be set in designating a suite of new kinds of type materials for new taxa. Phylogenetic analyses, which determine evolutionary relationships of taxa, will be run in ways unique to bacteria and uncommon in other groups. This work will help us to characterize biodiversity in the world. It may serve as an example for others wishing to use modern systematic approaches to describe new cyanobacterial species. Excellent systematics and taxonomy are necessary for development of the cyanobacteria for biotechnological, pharmacological, and reclamation purposes.

约翰·卡罗尔大学的 Jeffrey R. Johansen 博士获得了一笔资助，用于研究一种有问题的蓝绿藻（蓝藻）属，称为 Leptolyngbya。 该属很难理解，因为它的形态特征很少，但出现在多种栖息地类型中。 人们发现形态相似的形式实际上在 16S rRNA 基因序列方面有很大不同。 Johansen 博士将使用多相方法来表征来自世界各地的 60 多种微生物菌株，其中包括作为过去 NSF 支持项目的一部分而分离出的大量沙漠土壤形态。 多相表征意味着将收集这些菌株的形态、遗传、生态和生理数据，以便对属进行修订。 初步证据表明该属中有许多新物种，甚至可能有值得创建新属的物种群体。 与所有其他蓝藻分类学研究和大多数细菌学系统研究不同，该项目将测试三个现代物种概念对无性物种的适用性。 为新类群指定一套新型类型材料将开创更多先例。 确定类群进化关系的系统发育分析将以细菌特有的方式进行，而这在其他群体中并不常见。 这项工作将帮助我们描述世界生物多样性的特征。 对于其他希望使用现代系统方法来描述新蓝藻物种的人来说，它可以作为一个例子。 优秀的系统学和分类学对于用于生物技术、药理学和回收目的的蓝细菌的开发是必要的。