Polyphasic studies on exiccata: phylogenetic evaluation of botanical type materials from historical Herbaria for cyanobacteria

多相分类学将首次应用于蓝藻的历史植物标本库，整合了基因类型和表型研究。新的分子工具与经典的分类学程序相结合，有望改善这种代谢一致但形态复杂的微生物的分类基础。拟议的项目将向保存在草本植物中的经典描述的模式物种引入分子数据。这样，植物学意义上的模式物种也可以被微生物学家接受。在该项目期间获得的数据将使两个方面的统一迈出第一步：植物学和细菌学代码，至少在蓝藻的情况下是这样。在这里，我们建议引入基于分子方法和形态数据的多相分类系统，为积累历史上蓝藻草本植物的分类知识提供一个新的支架。此外，还将对现有的有毒或固氮菌株与形态相同的类型材料进行分子比较，以测试随着时间的推移基因类型的变化。该项目的新奇之处在于，将从遗传和表型属性方面探索模式标本。这打开了验证大量生态相关研究的可能性，并将现代多样性多相评估与传统的基于表型的鉴定和花卉名录联系起来。将首次测试由表型特征鉴定的微生物是否真的与传统假设的相同基因相对应。它还将测试依赖固定模式标本作为参考的生理学实践与以活的无菌培养作为类型参考的细菌学实践是更可靠的。

For the first time polyphasic taxonomy integrating genotypic and phenotypic studies will be applied to historical herbarium collections of cyanobacteria. The new molecular tools combined with classical taxonomic procedures promise to improve the basis for classification of this metabolically uniform but morphologically complex microorganisms. The project proposed will introduce molecular data to classically described type species deposited in Herbaria. In that way type species in botanical sense could be also accepted by microbiologists. Data gained during this project would allow doing the first step in unification of two: botanical and bacteriological codes, at least in case of cyanobacteria. Here, we propose to introduce polyphasic taxonomy system based on molecular methods and morphological data to provide a new scaffold for the accumulated taxonomic knowledge on historical cyanobacterial herbaria. Furthermore molecular comparison of present e.g. toxic or nitrogen fixing strains with the morphologically identical, type-material will be performed in order to test the genotypic changes over time. The novelty of the project is that a type specimen will be explored in terms of its genetic as well as phenotypic properties. This opens the possibility to validate a large volume of ecologically relevant research and connect the modern polyphasic assessment of diversity with traditional phenotype-based identifications and floral listings. It will be tested for the first time whether microorganisms identified by phenotypic characters indeed correspond to the same genotype as traditionally assumed. It will also test whether the phycological practice of relying on fixed type specimens for reference or the bacteriological one using live axenic cultures as type reference are more reliable.