Polyphasic studies on exiccata: phylogenetic evaluation of botanical type materials from historical Herbaria for cyanobacteria

第一次多相分类整合基因型和表型研究将适用于历史标本馆收集的蓝藻。新的分子工具结合经典的分类程序的承诺，以改善这种代谢均匀，但形态复杂的微生物的分类基础。建议的项目将把分子数据引入到保存在Herbaria的经典描述的模式物种中。这样，植物学意义上的模式种也可以被微生物学家接受。在这个项目中获得的数据将允许做两个统一的第一步：植物学和细菌学代码，至少在蓝藻的情况下。在这里，我们建议引入多相分类系统的分子方法和形态学数据的基础上积累的分类知识，历史蓝藻植物标本库提供了一个新的支架。此外，将对现有菌株（例如毒性或固氮菌株）与形态学相同的类型材料进行分子比较，以检测基因型随时间的变化。该项目的新奇之处在于，一个模式标本将在其遗传和表型特性方面进行探索。这为验证大量与生态相关的研究提供了可能性，并将现代多相多样性评估与传统的基于表型的鉴定和花卉清单联系起来。这将是第一次测试是否微生物鉴定的表型特征确实对应于相同的基因型，传统上认为。它还将测试依靠固定模式标本作为参考的藻类学实践或使用活的无菌培养物作为模式参考的细菌学实践是否更可靠。

For the first time polyphasic taxonomy integrating genotypic and phenotypic studies will be applied to historical herbarium collections of cyanobacteria. The new molecular tools combined with classical taxonomic procedures promise to improve the basis for classification of this metabolically uniform but morphologically complex microorganisms. The project proposed will introduce molecular data to classically described type species deposited in Herbaria. In that way type species in botanical sense could be also accepted by microbiologists. Data gained during this project would allow doing the first step in unification of two: botanical and bacteriological codes, at least in case of cyanobacteria. Here, we propose to introduce polyphasic taxonomy system based on molecular methods and morphological data to provide a new scaffold for the accumulated taxonomic knowledge on historical cyanobacterial herbaria. Furthermore molecular comparison of present e.g. toxic or nitrogen fixing strains with the morphologically identical, type-material will be performed in order to test the genotypic changes over time. The novelty of the project is that a type specimen will be explored in terms of its genetic as well as phenotypic properties. This opens the possibility to validate a large volume of ecologically relevant research and connect the modern polyphasic assessment of diversity with traditional phenotype-based identifications and floral listings. It will be tested for the first time whether microorganisms identified by phenotypic characters indeed correspond to the same genotype as traditionally assumed. It will also test whether the phycological practice of relying on fixed type specimens for reference or the bacteriological one using live axenic cultures as type reference are more reliable.