BAC Library: Diversity of Teleost Fishes

硬骨鱼是世界各地水生生态系统的主要组成部分，作为动物蛋白质的主要来源，具有巨大的商业价值。硬鱼在过去的200年里经历了惊人的适应性辐射。硬骨鱼有25,000种，占所有现存脊椎动物分类群的一半以上。鱼类是生物学研究的重要模型系统，从生态学、进化和发展到生物医学。为人类基因组测序而开发的基因组工具现在正在许多分类群中得到应用。三种鱼类（达尼欧鱼、河豚和四齿鱼）的基因组正在被完全测序。基因图谱已经建立了十几个其他物种，其中一些物种的物理图谱正在建设中。其他物种的BAC文库将进一步研究物种间的系统发育关系，类群间适应差异的遗传基础，以及调节基因组进化的模式和过程。本项目旨在通过构建和分布代表硬骨鱼主要谱系的两个物种的大插入基因组DNA文库，进一步促进鱼类的比较基因组研究。它们是盲洞鱼（Astyanax mexicanus）和马拉维湖斑马（Metriaclima zebra）。这些BAC文库将永久存档并通过UNH Hubbard基因组研究中心分发，该中心是一个专注于比较和环境基因组学的新基因组中心。每个文库将作为克隆分布在384孔板和适合杂交筛选的网格过滤器中。广泛的质量控制措施将确保每个衍生产品（复制板或滤镜）可以追溯到原始克隆。哈伯德中心致力于鱼类比较基因组资源的开发。该提案是建立鱼类基因组和cDNA文库、遗传和物理图谱以及比较图谱数据库计划的第一步。该文库将为今后系统发育、基因组进化、发育、进化、环境生物学和水产养殖等方面的研究奠定基础

0208343KocherTeleost fishes are dominant components of aquatic ecosystems around the world, and are of tremendous commercial importance as a major source of animal protein. Teleosts have undergone a spectacular adaptive radiation over the last 200MY. The 25,000 species of teleost represent more than half of all living vertebrate taxa. Fishes are important model systems across the spectrum of biological research, from ecology, evolution and development to biomedicine. The genomic tools developed to sequence the human genome are now finding applications across many taxa. The genomes of three fish species (Danio, Fugu and Tetraodon) are being completely sequenced. Genetic maps have been constructed for a dozen other species, and physical maps are under construction for a few of these. BAC libraries for additional species will further research aimed at discovering the phylogenetic relationships among species, the genetic basis for adaptive differences among taxa, and the patterns and processes that regulate genome evolution. This project aims to further stimulate comparative genomic studies of fishes by constructing and distributing large-insert genomic DNA (BAC) libraries for two species representing major teleost lineages. They are the blind cavefish (Astyanax mexicanus), and the Lake Malawi zebra (Metriaclima zebra). These BAC libraries will permanently archived and distributed through the UNH Hubbard Center for Genome Studies, a new genome center focused on comparative and environmental genomics. Each library will be distributed as clones in 384-well plates and gridded filters suitable for hybridization screening. Extensive quality control measures will ensure that each derived product (replica plate or filter) can be traced back to the original clone. The Hubbard Center is committed to the development of comparative genomic resources for fishes. This proposal is the first step in a program to generate genomic and cDNA libraries, genetic and physical maps, and comparative mapping databases for fishes. The libraries proposed here will serve as a foundation for future research in phylogenetics, genome evolution, development, evolution, environmental biology and aquaculture.__