BAC Library: Diversity of Teleost Fishes

KocherTeleost鱼是世界各地水生生态系统的主要组成部分，作为动物蛋白的主要来源具有巨大的商业价值。在过去的200年里，硬骨鱼经历了一次壮观的适应性辐射。25,000种硬骨鱼代表了所有现存脊椎动物种类的一半以上。从生态学、进化和发展到生物医学，鱼类是生物学研究范围内的重要模式系统。为人类基因组测序而开发的基因组工具现在正在许多分类群中找到应用。三种鱼(Danio、Fugu和Tetraodon)的基因组正在完成测序。已经为其他十几个物种构建了遗传图谱，其中几个物种的物理图谱正在构建中。更多物种的BAC文库将进一步研究，旨在发现物种之间的系统发育关系，分类群之间适应性差异的遗传基础，以及调控基因组进化的模式和过程。该项目旨在通过构建和分布代表主要硬骨鱼谱系的两个物种的大插入基因组DNA(BAC)文库，进一步促进鱼类的比较基因组研究。它们是盲穴居鱼(Astianax Micianus)和马拉维湖斑马(Metriaclima斑马)。这些BAC文库将通过UNH Hubbard基因组研究中心永久存档和分发，该中心是一个专注于比较和环境基因组学的新基因组中心。每个文库将以克隆的形式分布在适合杂交筛选的384孔板和网格过滤器中。广泛的质量控制措施将确保每个衍生产品(仿制版或过滤器)都可以追溯到原始克隆。哈伯德中心致力于鱼类比较基因组资源的开发。这项提议是建立鱼类基因组和cdna文库、遗传和物理图谱以及比较图谱数据库的计划的第一步。这些文库将为今后在系统发育、基因组进化、发育、进化、环境生物学和水产养殖等领域的研究奠定基础。

0208343KocherTeleost fishes are dominant components of aquatic ecosystems around the world, and are of tremendous commercial importance as a major source of animal protein. Teleosts have undergone a spectacular adaptive radiation over the last 200MY. The 25,000 species of teleost represent more than half of all living vertebrate taxa. Fishes are important model systems across the spectrum of biological research, from ecology, evolution and development to biomedicine. The genomic tools developed to sequence the human genome are now finding applications across many taxa. The genomes of three fish species (Danio, Fugu and Tetraodon) are being completely sequenced. Genetic maps have been constructed for a dozen other species, and physical maps are under construction for a few of these. BAC libraries for additional species will further research aimed at discovering the phylogenetic relationships among species, the genetic basis for adaptive differences among taxa, and the patterns and processes that regulate genome evolution. This project aims to further stimulate comparative genomic studies of fishes by constructing and distributing large-insert genomic DNA (BAC) libraries for two species representing major teleost lineages. They are the blind cavefish (Astyanax mexicanus), and the Lake Malawi zebra (Metriaclima zebra). These BAC libraries will permanently archived and distributed through the UNH Hubbard Center for Genome Studies, a new genome center focused on comparative and environmental genomics. Each library will be distributed as clones in 384-well plates and gridded filters suitable for hybridization screening. Extensive quality control measures will ensure that each derived product (replica plate or filter) can be traced back to the original clone. The Hubbard Center is committed to the development of comparative genomic resources for fishes. This proposal is the first step in a program to generate genomic and cDNA libraries, genetic and physical maps, and comparative mapping databases for fishes. The libraries proposed here will serve as a foundation for future research in phylogenetics, genome evolution, development, evolution, environmental biology and aquaculture.__