Upgrading the Core Facility for DNA Sequencing at the University of New Hampshire

升级新罕布什尔大学 DNA 测序核心设施

• 批准号: 9977187
• 负责人: Thomas Kocher
• 金额: $ 16.44万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9977187&HistoricalAwards=false
• 关键词: Upgrading; Core; Facility; DNA; Sequencing

Award Abstract 9977187 Funds will be used to purchase new automated DNA sequencing equipment with improved capabilities and higher throughput for the UNH core facility. This facility processes more than 17,000 samples per year which are submitted by the labs of some 22 faculty from 5 academic departments in the University. Major projects using the equipment include genome mapping of fishes, population genetics of fishes and zooplankton, and cDNA sequencing of vertebrate hormones. Additional studies are focussed on mechanisms of signal transduction in eucaryotic cells, invertebrate models of human leukemia, development of transgenic macroalgae, identification of coral symbionts, studies of marine invertebrate phylogeny, and sequencing of vertebrate visual pigment genes. Studies of gene structure, function, and evolution continue to drive demand for DNA sequencing in our research. New genomic approaches are becoming increasingly prevalent in all areas of biology, and have greatly increased the need for genotyping services at our facility in recent years. The new sequencing equipment will enhance the capability, increase the capacity, reduce the cost, and improve the reliability of sequencing services in the core facility at the University of New Hampshire.

9977187资金将用于为UNH核心设施购买新的自动化DNA测序设备，这些设备具有更高的能力和更高的吞吐量。 该设施每年处理超过17，000个样品，这些样品由来自大学5个学术部门的约22名教师的实验室提交。 使用该设备的主要项目包括鱼类基因组图谱、鱼类和浮游动物的群体遗传学以及脊椎动物激素的cDNA测序。 其他研究集中在真核细胞的信号转导机制，人类白血病的无脊椎动物模型，转基因大型藻类的发展，珊瑚共生体的鉴定，海洋无脊椎动物的遗传学研究，以及脊椎动物视觉色素基因的测序。对基因结构、功能和进化的研究继续推动我们研究中对DNA测序的需求。 新的基因组学方法在生物学的各个领域越来越普遍，近年来大大增加了我们机构对基因分型服务的需求。 新的测序设备将提高能力，增加容量，降低成本，并提高新罕布什尔州大学核心设施的测序服务的可靠性。