SBIR Phase I: Cavity-Enhanced Capillary Electrophoresis

SBIR 第一阶段：腔增强毛细管电泳

• 批准号: 0211557
• 负责人: Manish Gupta
• 金额: $ 10万
• 依托单位: LOS GATOS RESEARCH INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0211557&HistoricalAwards=false
• 关键词: SBIR; Phase; Cavity; Enhanced; Capillary

0211557GuptaThis Small Business Innovation Research Phase I Project proposes to apply cavity-enhanced spectroscopy to Capillary Electrophoresis (CE). CE typically relies on absorption spectroscopy to detect analytes eluting from a separation column. Although this detection method is very useful, the capillary's short absorptionpathlength has limited its sensitivity, forcing the development of more complex detection schemes. Preliminary results suggest that an optical cavity can be used to enhance this absorption sensitivity by a factor of hundred to over ten thousand. The aim of this proposal is to interface a CE column to an optical cavity and demonstrate the enhanced detection of various biological samples. Due to the increasing popularity of CE in biochemical research, the commercial applications of this technology continue to grow. It is currently used to analyze DNA fragments, proteins, and various drugs. Moreover, it is also being considered as an alternate to gel electrophoresis for DNA sequencing and is a leading prospect for lab-on-a-chip technologies. The commercial applications of this project are in genomic sequencing, medical diagnostics, and drug screening.

0211557Gupta这个小型企业创新研究第一阶段项目建议将腔增强光谱法应用于毛细管电泳（CE）。 CE 通常依靠吸收光谱来检测从分离柱洗脱的分析物。尽管这种检测方法非常有用，但毛细管的短吸收路径长度限制了其灵敏度，迫使开发更复杂的检测方案。初步结果表明，光学腔可用于将吸收灵敏度提高数百至一万倍。该提案的目的是将 CE 柱连接到光学腔，并演示各种生物样品的增强检测。由于毛细管电泳在生化研究中的日益普及，该技术的商业应用不断增长。它目前用于分析 DNA 片段、蛋白质和各种药物。此外，它还被认为是 DNA 测序凝胶电泳的替代方法，并且是芯片实验室技术的领先前景。该项目的商业应用包括基因组测序、医学诊断和药物筛选。