Functional Genomics of Host-Virus Interactions

真核病毒与其宿主之间的关系是大多数共同进化的病原体关系的特征。病毒与宿主相互作用的结果在遗传上是预先决定的。要产生疾病，病毒必须进入宿主，在宿主组织中局部繁殖，从进入部位扩散，克服或逃避宿主的免疫反应。植物进化出多种抗病毒防御策略来清除病毒感染。另一方面，病毒进化出了反防御策略。因此，了解病毒如何逃避宿主抗病毒防御的分子机制是很重要的。烟草摇铃病毒诱导的基因沉默（VIGS）系统将用于鉴定病毒抗性和易感因子的抑制因子。此外，功能蛋白质组学方法将用于研究病毒与宿主的相互作用。从这些研究中获得的知识将有助于防治植物传染性疾病。保护作物免受病害可以显著提高农业生产。利用植物自身的防御机制可以更有效地抵御植物病原体。利用在抗病信号通路中起作用的细胞基因来控制病原体引起的疾病，可能会提供巨大的农业效益，并通过提供一种替代农药的方法来预防疾病，从而为环境服务。在这个项目中开发的工具和信息将提供给科学界。这些资源将有助于改善经济上重要的植物，如西红柿、土豆和胡椒。可交付产品：pTRV1， pTRV2, pTRV2- gateway, pTRV-NbPDS， pTRV-tomPDS VIGS系统。通过9/03联系savithramma.dinesh-kumar@yale.eduAvailable: 250-300序列验证ptrv2 -番茄EST克隆，1000-1500序列验证pTRV2-Nb-cDNA农杆菌TMV-TAP载体

The relationship between eukaryotic viruses and their hosts are characteristic of mosthost-pathogen relationships that have co-evolved. The outcomes of virus-host interactions are genetically pre-determined. To produce disease, viruses must enter the host, multiply locally in host tissues, spread from the site of entry, and overcome or evade host immune responses. Plants have evolved various anti-viral defense strategies to clear viral infection. On the other hand, viruses have evolved counter-defense strategies. Therefore, it is important to understand molecular mechanisms of how viruses evade the host's antiviral defenses.A Tobacco Rattle Virus-based virus induced gene silencing (VIGS) system will be used to identify suppressors of viral resistance and susceptibility factors. In addition, a functional proteomics approach will be used to study virus-host interactions. The knowledge gained from these studies will help to combat infectious plant diseases. Protection of crops from disease can significantly improve agricultural production. Application of a plant's own defense mechanism can lead to more effective protection against plant pathogens. Control of pathogen-induced diseases using cellular genes that function in the disease resistance signaling pathway may provide tremendous agricultural benefits and serve the environment by offering an alternative to pesticide use to prevent disease. Tools and information developed in this project will be made available to the scientific community. These resources will assist in the efforts to improve economically important plants like tomato, potato and pepper.DeliverablesAvailable now: pTRV1, pTRV2, pTRV2-GATEWAY, pTRV-NbPDS, pTRV-tomPDS VIGS system. Contact savithramma.dinesh-kumar@yale.eduAvailable by 9/03: 250-300 sequence verified pTRV2-tomato EST clones 1000-1500 sequence verified pTRV2-Nb-cDNA Agrobacterium TMV-TAP vector