Functional Genomics of Host-Virus Interactions

宿主-病毒相互作用的功能基因组学

• 批准号: 0211872
• 负责人: Savithramma Dinesh-Kumar
• 金额: --
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0211872&HistoricalAwards=false
• 关键词: Functional; Genomics; Host; Virus; Interactions

The relationship between eukaryotic viruses and their hosts are characteristic of mosthost-pathogen relationships that have co-evolved. The outcomes of virus-host interactions are genetically pre-determined. To produce disease, viruses must enter the host, multiply locally in host tissues, spread from the site of entry, and overcome or evade host immune responses. Plants have evolved various anti-viral defense strategies to clear viral infection. On the other hand, viruses have evolved counter-defense strategies. Therefore, it is important to understand molecular mechanisms of how viruses evade the host's antiviral defenses.A Tobacco Rattle Virus-based virus induced gene silencing (VIGS) system will be used to identify suppressors of viral resistance and susceptibility factors. In addition, a functional proteomics approach will be used to study virus-host interactions. The knowledge gained from these studies will help to combat infectious plant diseases. Protection of crops from disease can significantly improve agricultural production. Application of a plant's own defense mechanism can lead to more effective protection against plant pathogens. Control of pathogen-induced diseases using cellular genes that function in the disease resistance signaling pathway may provide tremendous agricultural benefits and serve the environment by offering an alternative to pesticide use to prevent disease. Tools and information developed in this project will be made available to the scientific community. These resources will assist in the efforts to improve economically important plants like tomato, potato and pepper.DeliverablesAvailable now: pTRV1, pTRV2, pTRV2-GATEWAY, pTRV-NbPDS, pTRV-tomPDS VIGS system. Contact [e-mail] by 9/03: 250-300 sequence verified pTRV2-tomato EST clones 1000-1500 sequence verified pTRV2-Nb-cDNA Agrobacterium TMV-TAP vector

真核病毒及其宿主之间的关系是已共同发展的Mosthost-Pathot关系的特征。病毒宿主相互作用的结果是遗传预定的。为了产生疾病，病毒必须进入宿主，在宿主组织中局部繁殖，从进入部位扩散，并克服或逃避宿主免疫反应。植物已经进化了各种抗病毒防御策略，以清除病毒感染。另一方面，病毒已经发展了反防卫策略。因此，重要的是要了解病毒如何逃避宿主的抗病毒防御能力。基于烟灰反病毒的病毒诱导基因沉默（VIGS）系统将用于识别病毒抗性和易感性因素的抑制。另外，将使用一种功能蛋白质组学方法来研究病毒宿主相互作用。从这些研究中获得的知识将有助于消除传染性植物疾病。保护农作物免受疾病的影响可以显着改善农业生产。植物自己的防御机制的应用可以更有效地保护植物病原体。使用在疾病抗性信号传导途径中起作用的细胞基因对病原体诱导的疾病的控制可能会提供巨大的农业益处，并通过提供农药使用的替代方法来预防疾病，并为环境服务。该项目中开发的工具和信息将提供给科学界。这些资源将有助于改善经济重要的植物，例如番茄，马铃薯和胡椒。现在可以：PTRV1，PTRV2，PTRV2-GATEWAY，PTRV2-GATEWAY，PTRV-NBPD，PTRV-NBPD，PTRV-TOMPDS VIGS系统。联系[e-mail]可在9/03：250-300序列验证的PTRV2-Tomato est克隆1000-1500序列序列验证的PTRV2-NB-CDNA农杆菌TMV-TMV-TMV-TMV-TMV-TMV-TMV-TMV-TAP载体