EAGER: Small molecule regulation of plant autophagy and the biochemical characterization of whole intact autophagosomes

EAGER：植物自噬的小分子调控和完整自噬体的生化特征

• 批准号: 1355459
• 负责人: Savithramma Dinesh-Kumar
• 金额: $ 30万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-11-15 至 2015-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1355459&HistoricalAwards=false
• 关键词: EAGER; Small; molecule; regulation; plant

Autophagy, the regulated degradation and recycling of cellular material, is required for many cellular functions including the response to starvation, differentiation and development and senescence and immunity. However, the regulation of this process and the structure of the autophagosome (the organelle in which autophagy occurs) are not well understood in plants. This project will advance our understanding of autophagy in plants (specifically Arabidopsis) by addressing two major unsolved challenges 1) developing high-throughput assays to identify small chemical modulators of autophagy in plants, and 2) developing methods for the biochemical purification and study of plant autophagosomes. Broader Impacts. Training at the intersection of proteomics, biochemistry, cell biology and chemical genomics will be provided to a postdoctoral fellow and to undergraduate students.

自噬是细胞物质的受调节降解和回收，是许多细胞功能所必需的，包括对饥饿、分化和发育以及衰老和免疫的反应。然而，植物中这一过程的调节和自噬体（发生自噬的细胞器）的结构尚不清楚。该项目将通过解决两个尚未解决的主要挑战来增进我们对植物（特别是拟南芥）自噬的理解：1）开发高通量测定法来识别植物中自噬的小型化学调节剂，2）开发植物自噬体的生化纯化和研究方法。更广泛的影响。将为博士后研究员和本科生提供蛋白质组学、生物化学、细胞生物学和化学基因组学交叉领域的培训。