Evolution of CYP6B Genes Associated with Furanocoumarin Metabolism in the Papilio Glaucus Group

凤蝶群中与呋喃香豆素代谢相关的 CYP6B 基因的进化

• 批准号: 0212242
• 负责人: May Berenbaum
• 金额: $ 25万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0212242&HistoricalAwards=false
• 关键词: Evolution; CYP6B; Genes; Associated; Furanocoumarin

The vast majority of caterpillars feed on a narrow range of plants, often within only a single family. Among the distinguishing characteristics of such specialized feeders are enzymatic detoxification systems, including cytochrome P450 monooxygenases, that are ideally configured for metabolizing the toxins that typify their hostplants. The tiger swallowtail (Papilio glaucus) and its relatives, however, are exceptional among caterpillars in that they can feed on a much broader range of plant families. In doing so they encounter a substantially greater diversity of plant toxins, which differ in their biosynthetic origin and structure. This project is aimed at determining how the biochemistry and molecular biology of the cytochrome P450 monooxygenases of the tiger swallowtail and its generalist relatives differ from the P450 systems in their specialized relatives in the genus Papilio. Most Papilio species are specialized for feeding on plants containing toxins called furanocoumarins, and these species rely on P450s in the CYP6B family for detoxification. Although they rarely encounter them, tiger swallowtails can metabolize these compounds too, albeit at lower rates. In this project, differences in the structure and regulation of the CYP6B genes will be mapped within the genus Papilio (1) in order to understand how generalized habits can arise within specialized lineages and (2) to determine how P450 evolution can contribute to changes in detoxification capabilities.

绝大多数毛虫以范围很窄的植物为食，通常只在一个家庭内。 在这些专门的饲养者的显着特点是酶解毒系统，包括细胞色素P450单加氧酶，这是理想的配置代谢的毒素，代表他们的寄主植物。 然而，虎凤蝶（Papilio glaucus）及其亲属在毛虫中是例外的，因为它们可以以更广泛的植物家族为食。 在此过程中，它们遇到了种类繁多的植物毒素，这些毒素的生物合成来源和结构各不相同。本项目旨在确定虎燕尾及其通才亲属的细胞色素P450单加氧酶的生物化学和分子生物学与凤蝶属中其特化亲属的P450系统的差异。 大多数凤蝶物种专门以含有称为呋喃香豆素的毒素的植物为食，这些物种依赖于CYP6B家族中的P450进行解毒。 虽然它们很少遇到它们，但虎燕尾蝶也可以代谢这些化合物，尽管速度较低。 在这个项目中，CYP6B基因的结构和调控的差异将在凤蝶属中进行映射（1），以了解一般化的习惯如何在专门的谱系中出现，以及（2）确定P450进化如何有助于解毒能力的变化。