Regulation of Aromatic Compound Degradation in Acinetobacter

不动杆菌中芳香族化合物降解的调控

• 批准号: 0212604
• 负责人: Ellen Neidle
• 金额: $ 34万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2005-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0212604&HistoricalAwards=false
• 关键词: Regulation; Aromatic; Compound; Degradation; Acinetobacter

These studies focus on aromatic compound degradation by the soil bacterium Acinetobacter sp. strain ADP1. Catabolic pathways, which serve as model systems for understanding many aspects of biology, can be manipulated for bioremediation and for the generation of useful chemicals. The success of such ventures rests on a thorough understanding of microbial metabolic and genetic regulation. The first objective of this project is to determine the molecular basis for a recently discovered novel type of transcriptional activation. BenM, a transcriptional regulator involved in the degradation of benzoate by strain ADP1, was found to respond synergistically to two different metabolites. The synergistic modulation of transcriptional regulation in response to more than one inducer can significantly alter gene expression and may be a common biological regulatory feature. These studies will determine the specific nucleotide and amino acid sequences that control how BenM interacts with different effectors and DNA regions to regulate the expression of multiple genes and operons involved in aromatic compound catabolism. The second objective is to investigate preferential carbon source consumption by Acinetobacter strains when multiple growth substrates are available. Many soil bacteria degrade the components of a mixture of aromatic compounds in a sequential fashion. However, the method by which aromatic-compound degrading bacteria control the preferential consumption of carbon sources is unclear. In ADP1, regulation may occur at the transcriptional level, the post-transcriptional level, and/or at the level of substrate uptake into the cell. This research project will address how CatM and BenM repress genes needed for 4-hydroxybenzoate catabolism.

这些研究的重点是芳香族化合物降解土壤细菌不动杆菌属菌株ADP 1。 分解代谢途径作为理解生物学许多方面的模型系统，可以被操纵用于生物修复和产生有用的化学物质。 这种冒险的成功取决于对微生物代谢和遗传调节的透彻理解。这个项目的第一个目标是确定最近发现的一种新型转录激活的分子基础。 BenM是一种参与菌株ADP 1降解苯甲酸盐的转录调节因子，被发现对两种不同的代谢产物有协同反应。 响应于一种以上诱导物的转录调节的协同调节可以显著改变基因表达，并且可能是常见的生物学调节特征。 这些研究将确定特定的核苷酸和氨基酸序列，这些序列控制BenM如何与不同的效应子和DNA区域相互作用，以调节参与芳香族化合物催化剂的多个基因和操纵子的表达。第二个目标是调查不动杆菌菌株的优先碳源消耗时，多种生长底物。 许多土壤细菌以连续的方式降解芳香化合物混合物的组分。 然而，芳香族化合物降解菌控制碳源优先消耗的方法尚不清楚。 在ADP 1中，调控可能发生在转录水平、转录后水平和/或细胞内底物摄取水平。 该研究项目将解决CatM和BenM如何抑制4-羟基苯甲酸酯所需的基因。