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BRITE Future: Bioeconomy Relevant Innovation Through EASy

BRITE 未来：通过 EASy 进行生物经济相关创新

基本信息

批准号：
2225858
负责人：
Ellen Neidle
金额：
$ 50万
依托单位：
University of Georgia Research Foundation Inc
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-15 至 2025-07-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2225858&HistoricalAwards=false
关键词：
BRITE Future Bioeconomy Relevant Innovation

项目摘要

It is a national priority to accelerate biomanufacturing and to reduce dependence on refining petroleum to obtain commodity chemicals. This project focuses on developing techniques and bacterial strains that will allow industrially important compounds to be made from renewable resources rather than from fossil fuels. Towards this goal, the ability of bacteria to synthesize complex chemicals can be exploited. Among the petrochemicals in high demand are aromatic compounds that can be made by bacteria. However, natural biosynthetic pathways need to be modified to allow the accumulation of specific compounds at high levels to be economically feasible. This project involves the modification of a biosynthetic pathway in two kinds of soil bacteria, Acinetobacter baylyi and Pseudomonas putida to produce aromatic compounds. The research is accomplished through a collaboration with a team of scientists at Argonne National Laboratory. This collaboration has both scientific and educational components. Students learn about biomanufacturing and metabolic engineering, as well as careers in these fields, through a seminar series featuring scientists of the Agile BioFoundry. The long-term goal of this research is to create a cost-effective biosynthetic route for the bacterial conversion of plant-derived sugars to industrially needed aromatic compounds. Such compounds are in high demand and are commercially available now as petrochemicals. The bacteria employed in the research, Acinetobacter baylyi ADP1 and Pseudomonas putida KT2440, use the shikimate pathway to make aromatic compounds. To increase carbon flux into biosynthesis, an enzyme that is not typically part of the shikimate pathway is altered by novel mutagenesis and adaptive laboratory evolution methods and investigated in A. baylyi. This bacterium has an exceptionally high efficiency of natural transformation and allelic replacement and serves as host to generate novel enzymes. A modified shikimate pathway is engineered in P. putida using one of the novel enzymes generated in A. baylyi. This strategy uses the two different kinds of bacteria in a complementary approach that exploits unique features of each. The engineered pathway in P. putida is predicted to redirect carbon from a renewable biomass feedstock into high levels of desired end products. The collaboration with researchers from the Agile BioFoundry employs high throughput techniques to accomplish the project goals using advanced analytics and microfluidics-based techniques.This award is co-funded by the Systems and Synthetic Biology program in the Division of Molecular and Cellular Biosciences and the Cellular and Biochemical Engineering program in the Division of Chemical Bioengineering Environmental and Transport Systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

加快生物制造和减少对提炼石油以获得商品化学品的依赖是国家的优先事项。该项目的重点是开发技术和细菌菌株，使工业上重要的化合物能够从可再生资源而不是化石燃料中制备。为了实现这一目标，可以利用细菌合成复杂化学物质的能力。在石油化工产品中，需求量很大的是可以由细菌制造的芳香族化合物。然而，天然生物合成途径需要修改，以允许特定化合物在高水平的积累是经济可行的。本项目涉及两种土壤细菌，不动杆菌baylyi和恶臭假单胞菌的生物合成途径的修改，以产生芳香族化合物。这项研究是通过与阿贡国家实验室的一组科学家合作完成的。这种合作既有科学方面的，也有教育方面的。学生学习生物制造和代谢工程，以及在这些领域的职业生涯，通过一系列研讨会具有敏捷生物铸造的科学家。这项研究的长期目标是创造一种具有成本效益的生物合成途径，将植物来源的糖细菌转化为工业上需要的芳香族化合物。这种化合物的需求量很大，现在作为石油化学品可商购获得。在这项研究中使用的细菌，不动杆菌baylyi ADP 1和恶臭假单胞菌KT 2440，使用莽草酸途径来制造芳香化合物。为了增加进入生物合成的碳通量，通过新的诱变和适应性实验室进化方法改变通常不是莽草酸途径的一部分的酶，并在A. baylyi。该细菌具有非常高的天然转化和等位基因置换效率，并作为宿主产生新的酶。在恶臭假单胞菌中使用A. baylyi。这种策略以互补的方式使用两种不同的细菌，利用每种细菌的独特功能。预测恶臭假单胞菌中的工程化途径将来自可再生生物质原料的碳重定向为高水平的所需最终产物。与来自Agile BioFoundry的研究人员的合作采用高通量技术，使用先进的分析和基于微流体的技术来实现项目目标。由分子和细胞生物科学部的系统和合成生物学计划以及化学生物工程环境和运输系统部的细胞和生物化学工程计划资助。该奖项反映了NSF的法定基金会的使命是履行其使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评价，被认为值得支持。