EXPLORATORY RESEARCH ON ARCTIC MICROBIAL DIVERSITY

北极微生物多样性探索性研究

• 批准号: 0214231
• 负责人: Slava Epstein
• 金额: --
• 依托单位: Northeastern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-15 至 2003-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0214231&HistoricalAwards=false
• 关键词: EXPLORATORY; RESEARCH; ARCTIC; MICROBIAL; DIVERSITY

0214231EpsteinThe project is exploratory research on microbial diversity of Arctic sediments at two specific locations in Greenland. The effort will go to the field and work in the laboratory to- Investigate molecular diversity of marine sediment prokaryotes. Using total DNA extracts and 16S rRNA approach the PIs will characterize the Archaea and Bacteria found in the sediments at the experimental sites.- Examine molecular diversity of marine sediment Protista. They will characterize the genetic (18S rRNA and other genes) diversity of psychrophilic and psychrotolerant microeukaryotes at the experimental sites.- Explore in detail morphological and genetic diversity of one specific group of marine sediment protists, ciliated Protozoa. They will be looking for cryptic psychrophilic species that may remain un- or misudentified because of their morphological similarity to their relatives from the temperate areas.This exploratory project will lay the ground for the future large-scale research on ecology, evolution, genomics, and biotechnological potential of microorganisms from Arctic sediments.

0214231爱泼斯坦该项目是对格陵兰岛两个特定地点北极沉积物微生物多样性的探索性研究。这项工作将在实验室进行，以调查海洋沉积物原核生物的分子多样性。使用总DNA提取物和16S rRNA方法，PI将表征实验地点沉积物中发现的细菌和细菌。检查海洋沉积物原生生物的分子多样性。他们将描述实验地点嗜冷和耐冷微真核生物的遗传（18S rRNA和其他基因）多样性。详细探索海洋沉积物原生动物中一个特定群体的形态和遗传多样性，纤毛虫原生动物。他们将寻找可能由于其形态与温带地区的近缘种相似而未被确定或未被确定的神秘嗜冷种。这一探索性项目将为未来大规模研究北极沉积物中微生物的生态学、进化、基因组学和生物技术潜力奠定基础。