Acquisition of Gas Chromatography Equipment for Research in Microbiology and River Ecology at Three Undergraduate Institutions

为三个本科院校微生物学和河流生态学研究购置气相色谱设备

• 批准号: 0214978
• 负责人: David Glick
• 金额: $ 10.18万
• 依托单位: King's College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0214978&HistoricalAwards=false
• 关键词: Acquisition; Gas; Chromatography; Equipment; Research

A National Science Foundation grant has been awarded to King's College for microbiology laboratory equipment that will be used in undergraduate research at three local colleges. David Glick, Ph.D. and Brian Mangan, Ph.D. of King's College will share a new gas chromatography system installed at King's with Michael Sulzinski, Ph.D. of the University of Scranton and Kenneth Pidcock, Ph.D. of Wilkes University. The grant will demonstrate that shared use of equipment can foster the integration of research and education among undergraduate microbiology students and four highly skilled faculty investigators at three institutions of higher education.Two gas chromatographs configured with a computer and special software will facilitate new research in two areas. Separate, but related, research will identify and analyze lipids (fats) in bacteria, fungi, and fish through laboratory studies at the three institutions. Additional research will focus on Susquehanna River ecology, analyzing the effect of the environmental contaminant, polychlorinated biphenyl (PCB), on fish, and the effect of acid mine drainage on the abundance and diversity of river bacteria and fungi. One of the gas chromatographs will be dedicated to analyzing lipids (fats) extracted from bacteria and fungi. Once the lipid analysis has been done, the computer software supplied will allow the identification of the particular bacteria or fungus. The other gas chromatograph will be dedicated to the analysis of an important environmental contaminant known as PCB (Polychlorinated Biphenyl). This second instrument will also be used to examine which chemicals, in a mix, bacteria degrade.Prior to this NSF grant award, no institution of higher education in northeastern Pennsylvania possessed a gas chromatography system configured for research to identify and analyze microbes by their fatty acid profile. The research also has basic scientific importance. It will provide new insights into the ecology of soil and river bacteria and fungi as well as increasing our knowledge of the impact of PCB contamination on the life cycles of fish. The acquisition of this important equipment will strengthen the integration of undergraduate education, research and research training at all three institutions of higher education.

国家科学基金会向国王学院提供了一笔赠款，用于购买将用于三所当地学院本科研究的微生物学实验室设备。 大卫格利克博士和布莱恩·曼根博士将与迈克尔·苏尔津斯基博士共享安装在国王学院的新气相色谱系统。斯克兰顿大学和肯尼思·皮德科克博士威尔克斯大学 该赠款将表明，共享设备可以促进三所高等教育机构的本科微生物学学生和四名高技能教师调查员之间的研究和教育整合。两台配备计算机和特殊软件的气相色谱仪将促进新的研究在两个领域。 独立但相关的研究将通过三个机构的实验室研究来识别和分析细菌，真菌和鱼类中的脂质（脂肪）。 其他研究将集中在萨斯奎汉纳河生态，分析环境污染物，多氯联苯（PCB），对鱼类的影响，以及酸性矿山排水对河流细菌和真菌的丰度和多样性的影响。 其中一台气相色谱仪将专门用于分析从细菌和真菌中提取的脂质（脂肪）。一旦完成脂质分析，提供的计算机软件将允许识别特定的细菌或真菌。 另一台气相色谱仪将专门用于分析一种重要的环境污染物PCB（多氯联苯）。第二种仪器也将用于检测混合物中的哪些化学物质会被细菌降解。在获得NSF的资助之前，宾夕法尼亚州东北部的高等教育机构还没有一所拥有气相色谱系统，用于通过脂肪酸谱识别和分析微生物的研究。这项研究也具有基本的科学意义。它将为土壤和河流细菌和真菌的生态学提供新的见解，并增加我们对PCB污染对鱼类生命周期影响的了解。 购置这一重要设备将加强所有三所高等教育机构的本科教育、研究和研究培训的一体化。