SBIR Phase I: Overexpression of Membrane Proteins from Hyperthermophilic Bacteria by a Novel Expression System

该小型企业创新研究（SBIR）第一阶段项目将开发一种新型的膜蛋白表达系统，该系统使用能够合成大量膜蛋白并支持广泛的内部膜结构的超嗜热细菌。膜蛋白具有重要的药用价值。然而，研究膜蛋白的努力往往受到其生物合成水平低的阻碍。一个有效的膜蛋白过表达系统将有助于这些蛋白质的生物化学和生物物理特性。本项目将构建自复制型表达载体、基因组整合型表达载体和微型病毒基因组表达载体，并探索其表达途径。为了建立该系统的普遍适用性，将检查不同大小、结构和功能的各种膜蛋白的表达。该系统，一旦适当的开发，将允许经济的大规模生产的超嗜热膜蛋白的大规模结构基因组学的努力，以及为特定的工业应用。

This Small Business Innovation Research (SBIR) Phase I project will develop a novel membrane protein expression system using hyperthermophilic bacteria that are capable of synthesizing a vast amount of membrane proteins and supporting extensive internal membrane structures. Membrane proteins are of significant medicinal value. However, efforts to study membrane proteins are often hampered by their low level of biosynthesis. An efficient membrane protein overexpression system will facilitate the biochemical and biophysical characterization of such proteins. In this project, self-replicating expression vectors, genome integrative vectors and mini-viral genome vectors will be constructed and explored for the expression of hyperthermophilic membrane proteins. To establish the general applicability of the system, the expression of various membrane proteins of different size, structure, and function will be examined. The system, once properly developed, will allow the economical mass production of hyperthermophilic membrane proteins essential for large-scale structural genomics effort as well as for specific industrial applications.The commercial applications of this project are in the area of Structural Genomics Research.