SBIR Phase II: Overexpression of Membrane Proteins from Hyperthermophilic Bacteria - Refinement of a Novel Expression System

这个小企业创新研究（SBIR）第二阶段项目建议继续开发和改进一种新型的膜蛋白表达系统，该系统利用一组独特的细菌，能够合成大量的膜蛋白并支持广泛的内部膜结构。膜蛋白具有重要的药用价值。然而，研究膜蛋白的努力往往受到其生物合成水平低的阻碍。一个有效的膜蛋白过表达系统将有助于他们的生化和生物物理特性。这将允许大规模结构基因组学工作以及工业应用所必需的膜蛋白的经济大规模生产。该项目的商业影响将是生物技术和制药公司的药物发现工作。其他影响将在生物学和生理学领域，其中过程由膜蛋白调节（例如，农业）。

This Small Business Innovation Research (SBIR) Phase II Project proposes to continue the development and refinement of a novel membrane protein expression system utilizing a unique group of bacteria capable of synthesizing a vast amount of membrane proteins and supporting extensive internal membrane structures. Membrane proteins are of significant medicinal importance. However, efforts to study membrane proteins are often hampered by their low level of biosynthesis. An efficient membrane protein overexpression system will facilitate their biochemical and biophysical characterization. This will allow for the economical mass production of membrane proteins essential for large-scale structural genomics effort as well as for industrial applications. The commercial impact of the project will be on drug discovery work by biotechnology and pharmaceutical companies. Additional impact will be in areas of biology and physiology where processes are modulated by membrane proteins (for example, in agriculture).