SGER: Antibody-Conjugated Nanoparticle Films as Spectroscopic Sensors of Chemical Agents

0228143这项拟议的工作将制备坚固的、抗体偶联的金纳米颗粒薄膜，作为表面增强拉曼散射(SERS)的衬底，SERS是一种具有未被满足的无标记化学传感潜力的敏感光谱方法。基于纳米颗粒的衬底的表面增强拉曼散射活性将作为颗粒间距和周期有序的函数进行优化。将进行初步测试，以识别气体和水环境中的2，4-二硝基苯酚(DNP)，并在存在其他几种芳香族化合物的情况下作为选择性控制。用于选择性化学检测DNP的底物的演示可以立即用于开发基于拉曼的化学战剂传感器，包括炸药、神经毒气、芥子气和发泡剂。结构-活性关系仅基于理论考虑，没有实验上的优先权。然而，拟议的研究是假设驱动的，既涉及化学传感器开发的短期目标，也涉及纳米结构金属薄膜的等离子体性质的基本问题，随后有可能应用于化学和生物传感以外的领域。

0228143WeiThe proposed work will fabricate robust, antibody-conjugated gold nanoparticle films as substrates for surface-enhanced Raman scattering (SERS), a sensitive spectroscopic method with unmet potential for label-free chemical sensing. The SERS activities of the nanoparticle-based substrates will be optimized as a function of interparticle spacing and periodic order. Initial tests will be performed to recognize 2,4-dinitropohenol (DNP) in gas and aqueous environments and in the presence of several other aromatic compounds as a control for selectivity. The demonstration of substrates for selective chemical detection of DNP can be immediately parlayed into the development of Raman-based sensors of chemical warfare agents, including explosives, nerve gases, and mustard gases and vesicants. The structure-activity relationships are based on theoretical considerations alone and have no experimental precedence. However, the proposed research is hypothesis-driven and addresses both short-term objectives for chemical sensor development and fundamental issues on the plasmonic properties of nanostructured metal films, with subsequent potential for applications beyond chemical and biological sensing.