SGER: Antibody-Conjugated Nanoparticle Films as Spectroscopic Sensors of Chemical Agents

[0228143]该工作将制备健壮的抗体共轭金纳米颗粒薄膜作为表面增强拉曼散射（SERS）的底物，这是一种敏感的光谱方法，具有未满足的无标记化学传感潜力。纳米颗粒基基底的SERS活性将作为粒子间距和周期顺序的函数进行优化。将进行初步测试，以识别气体和水环境中的2,4-二硝基苯酚（DNP），以及存在几种其他芳香族化合物作为选择性控制。DNP选择性化学检测基质的演示可以立即应用于基于拉曼的化学战剂传感器的开发，包括炸药、神经毒气、芥子气和发泡剂。构效关系仅基于理论考虑，没有实验先例。然而，提出的研究是假设驱动的，解决了化学传感器发展的短期目标和纳米结构金属薄膜等离子体特性的基本问题，具有化学和生物传感以外的后续应用潜力。

0228143WeiThe proposed work will fabricate robust, antibody-conjugated gold nanoparticle films as substrates for surface-enhanced Raman scattering (SERS), a sensitive spectroscopic method with unmet potential for label-free chemical sensing. The SERS activities of the nanoparticle-based substrates will be optimized as a function of interparticle spacing and periodic order. Initial tests will be performed to recognize 2,4-dinitropohenol (DNP) in gas and aqueous environments and in the presence of several other aromatic compounds as a control for selectivity. The demonstration of substrates for selective chemical detection of DNP can be immediately parlayed into the development of Raman-based sensors of chemical warfare agents, including explosives, nerve gases, and mustard gases and vesicants. The structure-activity relationships are based on theoretical considerations alone and have no experimental precedence. However, the proposed research is hypothesis-driven and addresses both short-term objectives for chemical sensor development and fundamental issues on the plasmonic properties of nanostructured metal films, with subsequent potential for applications beyond chemical and biological sensing.