SGER: Antibody-Conjugated Nanoparticle Films as Spectroscopic Sensors of Chemical Agents

SGER：抗体偶联纳米粒子薄膜作为化学试剂的光谱传感器

• 批准号: 0228143
• 负责人: Alexander Wei
• 金额: $ 10万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0228143&HistoricalAwards=false
• 关键词: SGER; Antibody; Conjugated; Nanoparticle; Films

0228143WeiThe proposed work will fabricate robust, antibody-conjugated gold nanoparticle films as substrates for surface-enhanced Raman scattering (SERS), a sensitive spectroscopic method with unmet potential for label-free chemical sensing. The SERS activities of the nanoparticle-based substrates will be optimized as a function of interparticle spacing and periodic order. Initial tests will be performed to recognize 2,4-dinitropohenol (DNP) in gas and aqueous environments and in the presence of several other aromatic compounds as a control for selectivity. The demonstration of substrates for selective chemical detection of DNP can be immediately parlayed into the development of Raman-based sensors of chemical warfare agents, including explosives, nerve gases, and mustard gases and vesicants. The structure-activity relationships are based on theoretical considerations alone and have no experimental precedence. However, the proposed research is hypothesis-driven and addresses both short-term objectives for chemical sensor development and fundamental issues on the plasmonic properties of nanostructured metal films, with subsequent potential for applications beyond chemical and biological sensing.

0228143 Wei拟议的工作将制造坚固的，抗体共轭的金纳米粒子薄膜作为表面增强拉曼散射（Sers），一个敏感的光谱方法与未满足的潜力，无标记的化学传感基板。 基于纳米颗粒的基底的Sers活性将被优化为颗粒间间距和周期顺序的函数。 将进行初步测试，以识别气体和水环境中的2，4-二硝基苯酚（DNP），并在存在其他几种芳香族化合物的情况下作为选择性的对照。 DNP的选择性化学检测底物的演示可以立即用于开发基于拉曼的化学战剂传感器，包括爆炸物，神经毒气，芥子气和起泡剂。 结构-活性关系仅基于理论考虑，没有实验优先性。 然而，拟议的研究是假设驱动的，解决了化学传感器开发的短期目标和纳米结构金属薄膜等离子体特性的基本问题，随后有可能应用于化学和生物传感之外。