US-France Cooperative Research: Evolution and Function of Hopanoid Lipids in Symbiotic and Thermoacidophilic Actinomycetes

美法合作研究：共生和嗜热嗜酸放线菌中霍烷类脂质的进化和功能

• 批准号: 0233366
• 负责人: Alison Berry
• 金额: $ 1.52万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-15 至 2007-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0233366&HistoricalAwards=false
• 关键词: US; France; Cooperative; Research; Evolution

0233366BerryThis three-year award for US-France collaboration in microbial genetics involves Alison M. Berry of the University of California at Davis and Philippe Normand of the University of Claude Bernard - Lyon 1 and their students. The objectives are to study the biology of Frankia, a nitrogen-fixing prokaryote that forms root nodule symbioses and to address a key metabolic control point, the hopanoid lipid biosynthetic pathway. The production of specific hopanoid lipids in Frankia is a pivotal microorganism in that it is similar to the gram-negative rhizobia in its capacity to nodulate a group of related nitrogen-fixing plants. Frankia is related to thermoacidophilic Acidothermus cellulyticus. They will explore the role of hopanoids in symbiotic nitrogen fixation and as an adaptation to environmental stress through (1) DNA sequence information from the 17-kb Frankia genomic clone, and corresponding genes in close relatives: (2) comparisons of the profile of Frankia hopanoid lipids with that of A. Celluloyticus; and (3) environmental sampling of the thermoacidophilic actinomycetes, and expression of hopanoid genes. The information obtained in this collaboration will be used in expanded investigations of the evolution and function of hopanoid synthesis under extreme environmental conditions and by symbiotic signaling compounds. The US investigator brings to this collaboration expertise in hopanoid lipids and their importance in nitrogen fixation in Frankia. This is complemented by French expertise in Frankia phylogeny and in the use of molecular genetic tools for examining microbial communities in soils. This group also has the capacity to do proteomic analysis of Frankia. This award represents the US side of a joint proposal to the NSF and the French National Center for Scientific Research (CNRS). NSF will cover travel funds and living expenses for the US investigator and graduate students. The CNRS will support the visits of the French researchers and students to the United States. US students will benefit from the exchange of scientific techniques and experience with an international research team.

这个为期三年的美法微生物遗传学合作奖的获得者是加州大学戴维斯分校的艾莉森·m·贝瑞和克劳德·伯纳德-里昂大学的菲利普·诺曼和他们的学生。目的是研究Frankia的生物学，Frankia是一种形成根瘤共生的固氮原核生物，并解决一个关键的代谢控制点，藿烷类脂的生物合成途径。在法兰克菌中产生特定的藿烷类脂是一个关键的微生物，因为它与革兰氏阴性根瘤菌在结瘤一组相关固氮植物的能力相似。法兰克菌与嗜热嗜酸的胞酸菌有关。他们将通过以下途径探索hopanoid在共生固氮和适应环境胁迫中的作用：(1)Frankia基因组克隆的DNA序列信息，以及近亲的相应基因；(2)Frankia hopanoid脂质谱与A. Celluloyticus的比较；(3)嗜热嗜酸放线菌的环境采样及类藿烷基因的表达。在这次合作中获得的信息将用于扩大研究在极端环境条件下和通过共生信号化合物合成藿烷的进化和功能。美国研究者为这次合作带来了类hopanoid脂质的专业知识，以及它们在法兰克福固氮中的重要性。法国在法兰克植物系统发育和使用分子遗传学工具检查土壤微生物群落方面的专门知识对这一工作进行了补充。这个小组也有能力对法兰克虫进行蛋白质组学分析。该奖项代表了美国方面向美国国家科学基金会和法国国家科学研究中心（CNRS）提出的联合提案。美国国家科学基金会将支付美国研究人员和研究生的旅费和生活费。CNRS将支持法国研究人员和学生访问美国。美国学生将受益于国际研究团队的科学技术和经验交流。