Candidate Gene-Based Dissection of Butterfly Eyespot Evolution

蝴蝶眼斑进化的候选基因解析

• 批准号: 0235697
• 负责人: Anthony Long
• 金额: $ 76.7万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-15 至 2008-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0235697&HistoricalAwards=false
• 关键词: Candidate; Gene; Based; Dissection; Butterfly

The project will use cutting edge genomic techniques to dissect the genetic basis of butterfly wing eyespot patterning. These eyespots consist of concentric rings of different colors, and have important ecological roles in both predator avoidance and mate choice. Eyespots have long fascinated biologists and laymen alike. They are a particularly exciting model system as there is great diversity in these patterns within and between butterfly species and this diversity is amenable to analysis at different levels, including ecological, physiological, developmental, and genetical. The type of integrated approach that is possible with this system is of great value to biological research.We will develop genomic tools that will allow us to explore variation in the wing patterns of Bicyclus anynana butterflies. These tools will include a library containing genes which are expressed in developing butterfly wings, and a genetic map made using DNA sequence polymorphisms in many of those genes. The genetic map will help us localize the genes which contribute to eyespot variation. These tools will be a valuable asset for the larger scientific community working on Lepidopterans (butterflies and moths). Lepidopterans are still an understudied insect group despite their great positive (e.g., silkworms) and negative (e.g., many agricultural pests) economic impacts.

该项目将使用尖端的基因组技术来剖析蝴蝶翅膀眼斑图案的遗传基础。这些眼斑由不同颜色的同心环组成，在捕食者回避和配偶选择方面具有重要的生态作用。 眼点一直以来都让生物学家和外行人着迷。它们是一个特别令人兴奋的模型系统，因为蝴蝶物种内部和之间的这些模式具有很大的多样性，这种多样性可以在不同的层面上进行分析，包括生态学，生理学，发育和遗传学。该系统的综合方法对生物学研究具有重要价值，我们将开发基因组工具，使我们能够探索Bicyclus anynana蝴蝶翅膀图案的变化。这些工具将包括一个包含在发育中的蝴蝶翅膀中表达的基因的文库，以及利用许多这些基因中的DNA序列多态性制作的遗传图谱。基因图谱将帮助我们定位导致眼点变异的基因。这些工具将成为从事鳞翅目（蝴蝶和飞蛾）研究的更大科学界的宝贵财富。 鳞翅目昆虫仍然是一个研究不足的昆虫群体，尽管它们具有很大的积极意义（例如，蚕）和阴性（例如，农业害虫）的经济影响。