Structural Studies on the SSV1 Proteome; an Archeal Virus from an Extreme Thermal Environment

SSV1 蛋白质组的结构研究；

• 批准号: 0236344
• 负责人: Martin Lawrence
• 金额: $ 49.8万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0236344&HistoricalAwards=false
• 关键词: Structural; Studies; SSV1; Proteome; Archeal

Sulfolobus shibatae Virus (SSV) is a Crenarchaeal virus that exists in extreme thermal (greater than 80oC) and acidic (pH 3.0) environments such as the ones found in Yellowstone National Park. While SSV1 is the best characterized of any Crenarchaeal virus, the current understanding of these extremely unique viruses is very limited. Genomic sequence analysis of SSVs and most other viruses from extreme thermal environments fails to find homologues in the publicly available data bases for the vast majority of the putative open reading frames (ORFs). Thus, most of the SSV1 viral proteome, 30 of 34 ORFs, have yet to be assigned any function, and the roles that these proteins play in the viral life cycle remains a mystery. Using X-ray crystallography, this project seeks to identify structural homologues for member of the SSV1 proteome, thus providing insight into their possible function. The structural studies are being supplemented by the production of polyclonal antisera for each of these proteins, with subsequent analysis of their expression patterns and function with respect to the viral life cycle. Together, these data will begin to provide an understanding of the SSV1 life cycle at the molecular level. Broader Impact: Viruses of extreme thermophiles can serve as model systems for understanding the biochemistry and molecular biology required for life at high temperatures, thus this research will help to open a window onto some of the most fundamental processes of the still poorly understood Crenarchaeota. The structural biology investigated in this project will be incorporated in the undergraduate educational activities. Additionally, this project will provide outstanding training opportunities to students at different levels, including summer research experience for Native American students from Montana.

柴犬硫化叶病毒 (SSV) 是一种穹窿菌病毒，存在于极端高温（高于 80°C）和酸性（pH 3.0）环境中，例如黄石国家公园中发现的环境。 虽然 SSV1 是所有 Crearchaeal 病毒中特征最鲜明的，但目前对这些极其独特的病毒的了解非常有限。 对来自极端热环境的 SSV 和大多数其他病毒的基因组序列分析未能在公开数据库中找到绝大多数假定的开放阅读框 (ORF) 的同源物。 因此，大多数 SSV1 病毒蛋白质组（34 个 ORF 中的 30 个）尚未被指定任何功能，这些蛋白质在病毒生命周期中发挥的作用仍然是个谜。 该项目旨在利用 X 射线晶体学来鉴定 SSV1 蛋白质组成员的结构同源物，从而深入了解其可能的功能。 这些蛋白质的多克隆抗血清的生产对结构研究进行了补充，随后分析了它们的表达模式和病毒生命周期的功能。 总之，这些数据将开始在分子水平上提供对 SSV1 生命周期的理解。 更广泛的影响：极端嗜热病毒可以作为模型系统，用于了解高温下生命所需的生物化学和分子生物学，因此这项研究将有助于打开一扇窗户，了解目前仍知之甚少的Crenarchaeota的一些最基本的过程。本项目研究的结构生物学将纳入本科生教育活动。此外，该项目将为不同级别的学生提供出色的培训机会，包括为来自蒙大拿州的美国原住民学生提供暑期研究经验。