NMR-spektroskopische Untersuchungen der membranmodifizierenden Eigenschaften polyphiler Moleküle: Struktur und Lipiddynamik

多亲分子膜改性特性的核磁共振波谱研究：结构和脂质动力学

• 批准号: 161163426
• 负责人: Professor Dr. Kay Saalwächter, Ph.D.
• 金额: --
• 依托单位: Fachgruppe NMR
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/161163426?language=en
• 关键词: NMR; spektroskopische; Untersuchungen; der; membranmodifizierenden

The goal of this subproject is the comprehensive NMR-spectroscopic characterization of the membrane-modifying properties of polyphilic molecules which are synthesized in the subprojects of the other groups, and which are studied by complementary techniques in the groups of Blume and Bacia. Due to its chemical regio-selectivity, solid-state NMR, more than any other method, is well suited to clarify the origins of membrane-modifying properties on a molecular basis. In the first funding period, we were mainly occupied with methodological developments, subjecting different NMR methods to conceptual tests and widening their application range and the known limitations, and gained first insights into the interaction mechanisms. We have established methods that report on the one hand (i) on the structural arrangement of guest molecules at or in the membrane via the measurement of dipole-dipole couplings and cross relaxation/spin diffusion, and on the other hand (ii) on changes of the membrane properties such as stiffness, porosity, or defect content. For the latter, measurements of CH dipole-dipole couplings in the lipid chains, static 2H spectra of the interlamellar water, diffusion measurements of the through-membrane transport, and studies of the reorientation dynamics of the head groups via the 31P chemical shift anisotropy have proven useful. However in some cases, owing to partially contradictory and incomplete literature data, we see a further need for methodological development. A part of the studied polyphiles turned out to be merely surface active, i.e., small lamellar fragments (bicelles) were stabilized by lateral interactions and no changes in the molecular dynamic properties of the lipids were detected. Membrane-soluble polyphiles with distinct membrane-modifying properties were available only by the end of the period under report, and are currently subjected to a comprehensive study. First interesting results are presented in the project report

该子项目的目标是对在其他小组的子项目中合成的亲水分子的膜改性性质进行全面的NMR光谱表征，并通过Blume和巴西亚小组的互补技术进行研究。由于其化学区域选择性，固态NMR比任何其他方法更适合于在分子基础上澄清膜改性性质的起源。在第一个资助期内，我们主要致力于方法学的发展，对不同的NMR方法进行概念测试，扩大其应用范围和已知的局限性，并首次深入了解相互作用机制。我们已经建立了方法，一方面报告（i）通过偶极偶极耦合和交叉弛豫/自旋扩散的测量在膜上或膜中的客体分子的结构排列，另一方面报告（ii）膜性质的变化，如刚度，孔隙率或缺陷含量。对于后者，测量的CH偶极偶极耦合的脂质链，静态2 H光谱的层间水，通过膜运输的扩散测量，并通过31 P化学位移各向异性的头部基团的重定向动力学的研究已被证明是有用的。然而，在某些情况下，由于部分矛盾和不完整的文献数据，我们认为需要进一步的方法发展。一部分被研究的亲聚物被证明仅仅是表面活性的，即，小的层状片段（双胞）通过横向相互作用而稳定，并且没有检测到脂质的分子动力学性质的变化。具有不同膜改性特性的膜溶性亲聚物仅在本报告所述期间结束时可用，目前正在进行全面研究。第一个有趣的结果是在项目报告中提出的