CAREER: Biomolecular Engineering of Antigen Presentation: Development of an Interdisciplinary Research and Education Toolkit

职业：抗原生物分子工程演示：跨学科研究和教育工具包的开发

• 批准号: 0239099
• 负责人: Eric Boder
• 金额: $ 40万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0239099&HistoricalAwards=false
• 关键词: CAREER; Biomolecular; Engineering; Antigen; Presentation

The mammalian immune system demonstrates incredible power and flexibility in protection from disease and other organism processes, and the ability to stimulate controlled immune responses could revolutionize many areas of molecular medicine. Peptide antigen presentation to T cells by major histocompatibility complex proteins (MHC) is central to these immune responses; engineering MHC-peptide binding will require a detailed and quantitative understanding of the phenomena. This will require assiduous development of research tools and infrastructure for personnel training aimed at quantitative molecular design and optimization. The proposed work aims to (1) quantitatively study a related family of A related will be studied to quantitatively examine the kinetics and thermodynamics of binding between class II major histocompatibility complex protein (MHC-II) and a family of related peptide antigens; (2) elucidate correlations between peptide physicochemical properties and antigenic properties through computational structure analysis and functional studies; and (3) develop a directed evolution approach to tailoring MHC-II peptide-binding properties; (4) develop a graduate and undergraduate lecture and laboratory courses teaching state-of-art approaches in molecular biotechnology and biomolecular engineering.

哺乳动物的免疫系统在预防疾病和其他生物过程方面表现出令人难以置信的力量和灵活性，并且刺激受控免疫反应的能力可能会彻底改变分子医学的许多领域。 主要组织相容性复合体蛋白 (MHC) 将肽抗原呈递给 T 细胞是这些免疫反应的核心； MHC-肽结合工程需要对这种现象有详细和定量的了解。 这将需要努力开发研究工具和基础设施，以进行定量分子设计和优化的人才培训。 拟议工作的目的是（1）定量研究A相关的相关家族，以定量研究II类主要组织相容性复合体蛋白（MHC-II）与相关肽抗原家族之间结合的动力学和热力学； （2）通过计算结构分析和功能研究阐明肽理化性质与抗原性质之间的相关性； (3) 开发定向进化方法来定制 MHC-II 肽结合特性； (4) 开发研究生和本科生讲座和实验室课程，教授分子生物技术和生物分子工程的最新方法。