CAREER: Design of Self-Assembled Stimulus Responsive Peptide Complexes

职业：自组装刺激响应肽复合物的设计

• 批准号: 0239275
• 负责人: Alan Kennan
• 金额: $ 46万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0239275&HistoricalAwards=false
• 关键词: CAREER; Design; Self; Assembled; Stimulus

This research program addresses the design of self-assembled stimulus responsive peptide complexes. Alpha helical coiled coils consist of two or more helical strands, wrapped into a superhelix, which bind to one another through interactions between core hydrophobic and surface hydrophilic amno acid sidechains. By introducing unnatural amino acids in coiled coil contexts, these studies will generate more diverse sequences, allowing the design of complexes suitable for next-generation peptide hydrogels. By engineering the appropriate behavior into isolated peptide complexes, this research addresses the design of "smart" materials that respond to physiological stimuli (pH, temperature, salt gradients). A formal program in Chemical Biology will be established, providing for the recruitment and training of students at both the undergraduate and graduate levels in the fundamentals and applications of work in this area, bridging the gap between chemistry and biology.With the support of this CAREER Award from the Organic and Macromolecular Chemistry Program, Professor Alan Kennan, of the Department of Chemistry at Colorado State University, is carrying out fundamental studies related to the designed aggregation of proteins. By developing information about factors leading proteins to adopt particular structures, Professor Kennan designs assemblies of proteins that are expected to display unusual changes in response to their environment. These studies may lead to the development of "smart" materials that respond to physiological stimuli such as acidity, temperature and salt concentration. Professor Kennan will serve as the director of a new program in Chemical Biology, providing a variety of educational and support activities to recruit and train students in this interdisciplinary area, bridging the traditional disciplines of chemistry and biology.

本研究计划旨在设计自组装的刺激响应肽复合物。α螺旋卷曲螺旋由两个或多个螺旋链组成，缠绕成超螺旋，其通过核心疏水性和表面亲水性氨基酸侧链之间的相互作用彼此结合。通过在卷曲螺旋环境中引入非天然氨基酸，这些研究将产生更多样化的序列，允许设计适合下一代肽水凝胶的复合物。通过将适当的行为工程化到分离的肽复合物中，这项研究解决了对生理刺激（pH值，温度，盐梯度）做出反应的“智能”材料的设计。将建立一个正式的化学生物学计划，为本科生和研究生提供这一领域工作的基础和应用方面的招聘和培训，弥合化学和生物学之间的差距。在有机和高分子化学计划的职业奖的支持下，科罗拉多州立大学化学系的Alan Kennan教授，正在进行与蛋白质的设计聚集有关的基础研究。通过开发有关导致蛋白质采用特定结构的因素的信息，凯南教授设计了蛋白质的组装，这些蛋白质有望在响应环境时显示出不寻常的变化。这些研究可能会导致“智能”材料的发展，这些材料会对生理刺激做出反应，如酸度，温度和盐浓度。凯南教授将担任化学生物学新课程的主任，提供各种教育和支持活动，以招募和培养这个跨学科领域的学生，弥合化学和生物学的传统学科。