Uncovering the role of apocarotenoids in the arbuscular mycorrhiza symbiosis via mutation and knockdown of biosynthetic genes

通过生物合成基因的突变和敲低揭示类胡萝卜素在丛枝菌根共生中的作用

• 批准号: 161282083
• 负责人: Privatdozent Dr. Michael H. Walter
• 金额: --
• 依托单位: Abteilung Stoffwechsel- und Zellbiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/161282083?language=en
• 关键词: Uncovering; role; apocarotenoids; arbuscular; mycorrhiza

The arbuscular mycorrhiza(AM)-induced accumulation of certain apocarotenoids (C13 cyclohexenoneand C14 mycorradicin derivatives) is a ubiquitous phenomenon, whose importance and function in the AM symbiosis is being elucidated. Previous knockdown of two biosynthetic genes (DXS2 and CCD1) resulted in strong decreases of apocarotenoid accumulation correlated with an increase degenerating and dead arbuscules at the expense of mature ones. A current model on C13/C14 apocarotenoid mode of action proposing a role in a plantcontrolled degradation of nonfunctional arbuscules to optimize symbiotic performance and mutualism will be investigated further. A new target gene for pathway manipulation (CCD7) has emerged, which appears to constitute a crosspoint in C13/C14 and strigolactone hormone apocarotenoid biosynthesis. Mutants and knockdown transgenics for CCD7 and other pathway genes are available and additional ones will be generated and analyzed from Medicago truncatula. An AM-specific phosphate-transport mutant (mtpt4-1) producing nonfunctional arbuscules will be analyzed for a potential participation of apocarotenoids in its premature-degra-dation-of-arbuscules phenotype. Furthermore, additional downstream steps of apocarotenoid biosynthesis and metabolism will be identified and targeted for manipulation.

丛枝菌根（AM）诱导的某些脱辅基类胡萝卜素（C13环己烯酮和C14菌根素衍生物）的积累是一种普遍存在的现象，其在AM共生中的重要性和功能正在阐明。先前敲除两个生物合成基因（DXS 2和CCD 1）导致脱辅基类胡萝卜素积累的强烈减少，与成熟的代价增加退化和死亡的丛枝相关。目前的C13/C14脱辅基类胡萝卜素的行动模式提出了一个作用，在植物控制的非功能性arbuscules的降解，以优化共生性能和互惠关系的模型将进一步研究。一个新的靶基因的途径操纵（CCD 7）已经出现，这似乎构成了C13/C14和独脚金内酯激素脱辅基类胡萝卜素生物合成的交叉点。CCD 7和其他途径基因的突变体和敲低转基因是可用的，并且将从蒺藜苜蓿中产生和分析其他转基因。将分析AM特异性磷酸盐转运突变体（mtpt 4 -1）产生无功能丛枝的脱辅基类胡萝卜素在其过早降解丛枝表型中的潜在参与。此外，脱辅基类胡萝卜素的生物合成和代谢的其他下游步骤将被确定和有针对性的操作。

项目成果

Evolution of root-specific carotenoid precursor pathways for apocarotenoid signal biogenesis.

• DOI: 10.1016/j.plantsci.2014.12.017
• 发表时间: 2015-04
• 期刊: Plant science : an international journal of experimental plant biology
• 作者: M. Walter;R. Stauder;A. Tissier

Differential spatio-temporal expression of carotenoid cleavage dioxygenases regulates apocarotenoid fluxes during AM symbiosis.

• DOI: 10.1016/j.plantsci.2014.10.010
• 发表时间: 2015
• 期刊: Plant science : an international journal of experimental plant biology
• 作者: J. A. López-Ráez;I. Fernández;Juan M. García;Estefanía Berrio;P. Bonfante;M. Walter;M. Pozo