Development of Infrared Spectroscope for Microfabricated Proteome Chips

微加工蛋白质组芯片红外光谱仪的研制

• 批准号: 0242697
• 负责人: Shyamsunder Erramilli
• 金额: $ 59.77万
• 依托单位: Trustees of Boston University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0242697&HistoricalAwards=false
• 关键词: Development; Infrared; Spectroscope; Microfabricated; Proteome

This award supports development of an infrared spectroscope capable of observing the intrinsic molecular vibrations of protein complexes with the potential for both high sensitivity and high throughput. The instrument will use evanescent waves, obtained by use of a tunable laser operating in the mid-infrared, to examine proteins positioned on a microfabricated array of silicon cantilevers. Each cantilever has a solid immersion lens (SIL) on one side, and a site for protein deposition on the other. The deposition site will typically consist of a chemically-bound, known peptide plus one or more proteins bound only through an interaction with the peptide. The array will be positioned on the stage of a microscope. Use of infrared allows detection in the amide I band, where the peptide bond has a sufficiently large cross section to obviate the need for labeling of the protein. The project is an interdisciplinary collaboration between scientists and engineers at Boston University, Stanford University and the University of California at Berkeley. The instrument is expected to have applications in both basic and applied research, including studies aimed at improved understanding of the relationship between protein structure and function, and rapid screening of protein mixtures. The broader impacts of the project will involve the training of graduate students and periodic presentations to area high school students as part of an ongoing outreach program aimed at dissemination of scientific and technological understanding. These presentations occur during the school year and the summer as well. In addition, another ongoing effort at involving minority undergraduate women majoring in science and medical science will benefit.

该奖项支持开发能够观察蛋白质复合物内在分子振动的红外光谱仪，具有高灵敏度和高通量的潜力。该仪器将使用倏逝波，通过使用可调谐激光器在中红外操作获得，以检查位于硅微阵列上的蛋白质。每个悬臂在一侧具有固体浸没透镜（SIL），并且在另一侧具有用于蛋白质沉积的位点。 沉积位点通常由化学结合的已知肽加上仅通过与肽相互作用结合的一种或多种蛋白质组成。 将阵列定位在显微镜的载物台上。 红外线的使用允许在酰胺I带中进行检测，其中肽键具有足够大的横截面以避免需要标记蛋白质。 该项目是波士顿大学、斯坦福大学和加州大学伯克利分校的科学家和工程师之间的跨学科合作。该仪器有望应用于基础和应用研究，包括旨在更好地了解蛋白质结构和功能之间关系的研究，以及蛋白质混合物的快速筛选。该项目的更广泛影响将涉及培训研究生和定期向地区高中学生介绍情况，作为旨在传播科学和技术知识的持续推广方案的一部分。 这些介绍发生在学年和夏季以及。此外，另一项正在进行的让主修科学和医学的少数族裔本科女性参与的努力也将受益。