Geomicrobiology of a high saline mine tailings in the desert Atacama, Chile

应全面调查智利阿塔卡马沙漠Bahía de Chañaral湾高盐度硫化尾矿矿床的地质微生物学和微生物生态。利用催化报告沉积-荧光原位杂交(CARD-FISH)和实时定量聚合酶链式反应(Q-PCR)对5个采样点的微生物群落进行了深度相关的定量分析，旨在了解干旱气候高盐度下Fe(II)和硫氧化、Fe(III)和硫酸盐还原过程中的生物地球化学相互作用。利用变性梯度凝胶电泳法(DGGE)和16S rRNA基因测序对尾矿微生物多样性进行深度相关性分析，旨在鉴定高盐度尾矿中的相关微生物。为了能够在高盐度的水中进行生物浸出，将对尾矿中的耐盐性Fe(II)氧化微生物的富集物和菌株进行分析，并将其应用于不同盐浓度下的黄铁矿和黄铜矿的生物浸出试验。

The geomicrobiology and microbial ecology of a high saline, sulfidic mine tailings deposit in the bay Bahía de Chañaral, Atacama desert, Chile shall be comprehensively investigated. A depth dependent quantitative microbial community analysis for five sampling sites using catalyzed reporter deposition - fluorescence in situ hybridisation (CARD – FISH) and quantitative real-time PCR (Q-PCR) aims to understand the biogeochemical interactions during Fe(II)- and sulfuroxidation and Fe(III)- and sulfate-reduction at high salinity in arid climate. The depth dependent analysis of the microbial diversity using denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing of 16S rRNA genes aims to identify the relevant microorganisms in the high saline mine tailings. To enable biomining with water of high salinity, enrichments and isolates of halotolerant Fe(II)-oxidizing microorganisms from the tailings will be analysed and applied in bioleaching experiments with pyrite and chalcopyrite at varying salt concentrations.