Deep Biosphere Quantification in Chesapeake Bay Impact Structure Sediments

该项目将调查切萨皮克湾撞击结构撞击后沉积物的深层生物圈。主要目标是活的原核生物的可视化和量化以及丰富的原核生物群的量化。利用定量PCR和CARD-FISH技术，在太平洋深海沉积物中发现了大量的活细菌，占原核细胞总数的很大比例。我们计划使用这些技术，使这一重要的发现外推到切萨皮克湾的影响结构沉积物。定量PCR还将用于从丰富的16 S rDNA和功能基因簇（例如，硫酸盐还原原核生物的异化亚硫酸盐还原酶基因（dsrAB））中定量芽孢杆菌和细菌。此外，烃降解微生物将被富集，分离和描述。将分析来自不同深度的50个沉积物岩心样本，以探索“深层生物圈”。

The project will investigate the Deep Biosphere of the Chesapeake Bay Impact Structure post-impact sediments. The main objective is the visualization and quantification of living prokaryotes and the quantification of abundant prokaryotic groups. A high proportion of the total number of prokaryotic cells (AODC) has been identified as living Bacteria in deep sediments from the Pacific using quantitative PCR and CARD - FISH. We plan to use these techniques to allow the extrapolation of this important finding to the Chesapeake Bay impact structure sediments. Quantitative PCR will also be used to quantify Archaea and Bacteria from abundant clusters of 16S rDNA and functional genes, e.g. the dissimilatory sulfite reductase gene (dsrAB) of sulfate reducing prokaryotes. In addition, hydrocarbon-degrading microorganisms will be enriched, isolated and described. 50 sediment core samples from various depths will be analyzed to explore the "Deep Biosphere".