Cultivation and quantification of microorganisms in Baltic Sea deep sediments in response to glacial and interglacial cycles (IODP Exp. 347)

2013年9月至10月进行的国际海洋学项目第347次考察波罗的海盆地古环境，将冰川间冰期循环的深层生物圈反应作为四个主要研究主题之一。作为航行后研究的一部分，本DFG项目将检验以下假设：波罗的海深埋海洋沉积物中富集的微生物培养物和定量微生物群落组成在（1）湖沼、半咸水和海洋条件，（2）好氧和亚氧/缺氧条件，（3）低温和温和温度，或（4）低有机沉积和高有机沉积之间变化，反映在不同的沉积物芯样中。应使用分子定量技术实时PCR（qPCR）和催化报告沉积荧光原位杂交（CARD-FISH）以及培养和稳定同位素探测来揭示不同的微生物群落，并分离和描述新微生物。

IODP Expedition 347, Baltic Sea Basin Paleoenvironment, carried out in September-October 2013 addresses Deep biosphere responses to glacial interglacial cycles as one of four main research themes. This DFG project, as part of the post-cruise research, shall test the hypothesis that enriched microbial cultures and the quantitative microbial community composition in Baltic Sea deeply buried marine sediments vary between (1) limnic, brackish, and marine conditions, (2) oxic and suboxic/anoxic conditions, (3) low and temperate temperature, or (4) low and high organic sedimentation, reflected in the different sediment core samples. The molecular quantitative techniques real-time PCR (qPCR) and catalyzed reporter deposition fluorescence in situ hybridisation (CARD - FISH) as well as cultivation and stable isotope-probing shall be used to reveal the different microbial communities and to isolate and describe novel microorganisms.