Mechanical Properties of Living Cells: Atomic Force Microscopy Studies, Modeling, and Education

活细胞的机械特性：原子力显微镜研究、建模和教育。J. BERGER*， K. S. VEMAGANTI*, AND H. youc .辛辛那提大学，辛辛那提，45221提案cm -0301463摘要这项为期三年的NSF奖励支持活细胞力学特性测定的基础研究。辛辛那提大学（UC）工程学院和医学院的研究人员组建了一个跨学科团队，通过原子力显微镜（AFM）检查活细胞的压痕，以确定细胞的力学特性。该程序通过详细的AFM实验和全面的分析和数值模拟相结合，直接解决了当前细胞力学性能测定分析方法的许多局限性。这项工作的智力价值在于，它将提高从原子力显微镜压痕数据中提取活细胞力学特性的能力。这些信息将有助于实现理解细胞机械变形和细胞行为之间联系的长期研究目标。通过将这些改进的建模方法和AFM实验整合到一个双层次、跨学科的细胞力学课程中，可以实现更广泛的影响。

MECHANICAL PROPERTIES OF LIVING CELLS: ATOMIC FORCE MICROSCOPY STUDIES, MODELING, AND EDUCATIONE. J. BERGER*, K. S. VEMAGANTI*, AND H. YOUUNIVERSITY OF CINCINNATI, CINCINNATI OH, 45221PROPOSAL CMS-0301463AbstractThis three-year NSF award supports fundamental research in mechanical property determination of living cells. Researchers from the Colleges of Engineering and Medicine at the University of Cincinnati (UC) have assembled an interdisciplinary team to examine indentation of living cells via atomic force microscopy (AFM) for the purpose of determining cell mechanical properties. This program directly addresses many of the limitations of current analysis approaches for cell mechanical property determination through a combination of detailed AFM experiments and comprehensive analytical and numerical modeling. The intellectual merit of this work is that it will advance the ability to extract mechanical properties of living cells from AFM indentation data. This information will allow pursuit of the long-term research goal of understanding the link between cell mechanical deformations and cell behavior. Broader impact is achieved by integrating these improved modeling approaches and the AFM experiments into a dual-level, interdisciplinary Cellular Mechanics course.