CAREER: Micromechanics and Metabolic Properties of Living Interfacial Materials

职业：活性界面材料的微观力学和代谢特性

• 批准号: 2422153
• 负责人: Tagbo Niepa
• 金额: $ 66.34万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2422153&HistoricalAwards=false
• 关键词: CAREER; Micromechanics; Metabolic; Properties; Living

This award is funded in whole or in part under the American Rescue Plan Act of 2021 (Public Law 117-2).This Faculty Early Career Development (CAREER) award will support research to reveal how bacteria grow and adapt at the interface of water and oil, and at the interface of water and air. Films of bacterial aggregates, also called biofilms, are a ubiquitous form of microbial life. When they grow on solid-liquid interfaces, they can cause health problems like infections near joint implants. When biofilms grow at air-liquid interfaces, they can cause lung problems. How biofilms grow and adapt at these interfaces is not well understood. This work will first explore how bacteria cope with changes in surface tension and energy. Next, this work will study how bacteria’s adaptation to changing conditions can be manipulated to create new materials. Finally, this work will suggest how viruses and nanomaterials could be used to control bacterial development at fluid interfaces. The results of this work will ultimately be relevant for treating chronic lung infections or developing more effective treatment of crude oil spills using bacteria. Moreover, these research activities will motivate students to pursue STEM careers. The project will adapt professional engagement strategies to develop pre-college and college experiences for minorities, first-generation, and financially challenged students. It will promote an inclusive climate and facilitate these students’ academic success through a range of mentored experiences. Educational activities include a “Bugs as Materials” Camp, a college application workshop, and a summer experience for undergraduates.The physicochemical mechanisms that regulate microbial growth in biofilms remain poorly understood, in part because of the versatility of microorganisms’ ability to respond to diverse environmental conditions. Even less well-known are the mechanisms governing the growth and metabolic responses of biofilms formed at the fluid interface. To test the overarching hypothesis that bacteria metabolize a patch of an interface and secrete a protective coating to thrive under harsh interfacial conditions, three objectives will be pursued: (1) systematically elucidate the viscoelastic properties and the physiology of interfacial films; (2) characterize the effects of phenotypic adaptation of bacteria under interfacial confinement on interfacial film mechanics; and (3) determine how the mechanical integrity of mixed interfacial films is altered by chemical and biological insults. Model organisms, including Pseudomonas aeruginosa and Staphylococcus aureus, will help elucidate how films at bacterial interfaces form, and how the rheological properties are altered by physical, chemical, and biological insults.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项全部或部分根据2021年美国救援计划法案（公法117-2）资助。该教师早期职业发展（CAREER）奖将支持研究揭示细菌如何在水和油的界面生长和适应，以及在水和空气的界面。 细菌聚集体的膜，也称为生物膜，是微生物生命的普遍存在形式。当它们在固液界面上生长时，它们会导致健康问题，如关节植入物附近的感染。当生物膜在空气-液体界面生长时，它们会导致肺部问题。 生物膜如何在这些界面生长和适应还没有很好的理解。 这项工作将首先探索细菌如何科普表面张力和能量的变化。 接下来，这项工作将研究如何操纵细菌对不断变化的条件的适应来创造新材料。 最后，这项工作将建议如何使用病毒和纳米材料来控制流体界面处的细菌发育。 这项工作的结果最终将与治疗慢性肺部感染或开发使用细菌更有效地处理原油泄漏有关。此外，这些研究活动将激励学生追求STEM职业。该项目将调整专业参与战略，为少数民族，第一代和经济困难的学生开发大学预科和大学经验。它将促进一种包容性的气氛，并通过一系列辅导经验促进这些学生的学业成功。教育活动包括一个“昆虫作为材料”营，一个大学申请工作坊，和一个本科生暑期体验。调节生物膜中微生物生长的物理化学机制仍然知之甚少，部分原因是微生物对不同环境条件反应能力的多样性。更不为人所知的是在流体界面处形成的生物膜的生长和代谢反应的机制。为了验证细菌在界面上代谢并分泌保护性涂层以在苛刻的界面条件下茁壮成长的总体假设，将追求三个目标：（1）系统地阐明界面膜的粘弹性和生理学特性;（2）表征界面限制下细菌表型适应对界面膜力学的影响;以及（3）确定混合界面膜的机械完整性如何被化学和生物损伤改变。模式生物，包括铜绿假单胞菌和金黄色葡萄球菌，将有助于阐明薄膜在细菌界面的形成，以及如何流变学性质被改变的物理，化学和生物insuls.This奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。