Synthesis and Characterization of Fluorescent Porphyrinoid Bioconjugates for Imaging and Bioanalyses

用于成像和生物分析的荧光类卟啉生物共轭物的合成和表征

• 批准号: 0304833
• 负责人: Maria Vicente
• 金额: $ 75万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0304833&HistoricalAwards=false
• 关键词: Synthesis; Characterization; Fluorescent; Porphyrinoid; Bioconjugates

Maria Graca Vicente, Robert P. Hammer and Steven Soper, Department of Chemistry, Louisiana State University, are funded with an award from the Collaborative Research in Chemistry Program with funds provided by the Division of Chemistry, the Division of Molecular and Cellular Biology and the Office of Multidisciplinary Activities. This multifaceted team will investigate synthetic routes to novel water-soluble unsymmetrically-substituted tetrabenzoporphyrins and phthalocyanines, their chemical and photophysical characterization and conjugation to biomolecules. The use of these new biomarkers should contribute to an in-depth understanding of the structural factors controlling the effectiveness of novel bioimaging and bioanalytical probes. Tetrabenzoporphyrin and phthalocyanine These macrocycles have unique chemical, photophysical and solubility properties, and offer unique functionalization for conjugation to biomolecules such as proteins, nucleic acids and peptides.,. This synergistic collaboration will involve synthesis, characterization and application of unique porphyrinoid materials to develop improved fluorescent biomarkers, or fluorophores. In addition, students, including postdoctoral associates, graduate students and undergraduates will receive interdisciplinary training in modern bioimaging and bioanalytical science.

路易斯安那州立大学化学系的Maria Graca Vicente、Robert P.Hammer和Steven Sper获得了化学合作研究项目的资助，资金来自化学系、分子和细胞生物学系以及多学科活动办公室。这个多方面的团队将研究新型水溶性不对称取代的四苯并卟啉和酞菁的合成路线，它们的化学和光物理表征以及与生物分子的偶联。这些新生物标志物的使用应该有助于深入了解控制新型生物成像和生物分析探针有效性的结构因素。四苯并卟啉和酞菁这些大环具有独特的化学性质、光物理性质和溶解性，为蛋白质、核酸和多肽等生物分子的偶联提供了独特的官能化作用。这种协同合作将涉及独特的类卟啉材料的合成、表征和应用，以开发改进的荧光生物标记物或荧光团。此外，包括博士后助理、研究生和本科生在内的学生将接受现代生物成像和生物分析科学的跨学科培训。