MRI: Laboratory Instrumentation for Biophotonics: Investigation of Plant and Cyanobacteria Pigments

MRI：生物光子学实验室仪器：植物和蓝藻色素的研究

• 批准号: 0304798
• 负责人: Susan Spiller
• 金额: --
• 依托单位: Mills College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0304798&HistoricalAwards=false
• 关键词: MRI; Laboratory; Instrumentation; Biophotonics; Investigation

0304798SpillerThe requested instrumentation will facilitate the participation of PI, undergraduate women and post baccalaureate students, and faculty at Mills College, Oakland, CA in two research projects and increase potential for future projects. The general descriptions of the current projects are: 1) Control of yellow starthistle achene germination by phytochrome pigment 2) Relative pigment composition of two cyanobacteria genera, Spirulina and Arthrospira, compared to chlorophyte alga of the Spirogyra genus. A spectroradiometer plus LED and tunable laser illuminating instruments will enable us to deliver precise doses of photons in narrow bandwidths and to calibrate our systems precisely. The combined instrumentation will also allow us to analyze the absorbance characteristics of our experimental organisms. Our phytochrome research aims to obtain results that add to a body of knowledge about phytochrome, a plant pigment that controls developmental processes. Phytochrome acts as a switching mechanism whereby red light (660nm) initiates developmental processes by a conformation change in the chromophore and apoprotein. The initiating signal can be reversed by light wavelengths in the far red (730 nm) range by returning the chromophore and its attached apoprotein to the original conformation. The escape time from reversal of the developmental signal is one goal of our project. The phytochrome system is a very interesting biological switching mechanism with potential for useful applications in many areas of agriculture and medicine.

0304798 Spiller所要求的仪器将促进PI、本科女性和学士后学生以及加利福尼亚州奥克兰米尔斯学院的教师参与两个研究项目，并增加未来项目的潜力。目前的项目的一般描述是：1）控制黄色星蓟瘦果萌发的光敏色素色素2）相对色素组成的两个蓝藻属，螺旋藻和节旋藻，比较绿藻属水绵。 光谱辐射计加上LED和可调谐激光照明仪器将使我们能够在窄带宽内提供精确剂量的光子，并精确校准我们的系统。 组合的仪器也将使我们能够分析我们的实验生物体的吸收特性。 我们的光敏色素研究旨在获得增加有关光敏色素的知识体系的结果，光敏色素是一种控制发育过程的植物色素。 光敏色素作为一种转换机制，红光（660 nm）通过改变发色团和脱辅基蛋白的构象来启动发育过程。 起始信号可以通过使发色团及其附着的脱辅基蛋白返回到原始构象而被远红（730 nm）范围内的光波长逆转。 从发育信号逆转的逃逸时间是我们项目的一个目标。 光敏色素系统是一种非常有趣的生物转换机制，在农业和医学的许多领域具有有用的应用潜力。